CD3e (T-Cell Marker) (OKT3), 1mg/mL

About this product

Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28 kDa. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation. It is found on all mature T-lymphocytes, NK, T-cells, and some thymocytes, and is expressed, typically at high levels, on peripheral T cells and majority of T cell neoplasms. Thymocytes express CD3 at different level on the cell surface in the course of differentiation and CD3 is predominantly intracytoplasmic in cortical thymus. Anti-CD3 clone OKT3 has been reported to have potent immunosuppressive properties in vivo and has been proven effective in the treatment of renal, heart and liver allograft rejection. In the crystal structure of the antibody-receptor complex, clone OKT3 was found to bind a conformational epitope on CD3e dominated by a linear stretch in the F-G loop of the subunit. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNUM0268-50

Size / count

1x 50 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

CD3 epsilon; CD3 TCR complex; CD3e antigen epsilon polypeptide (TiT3 complex) ; T cell antigen receptor complex epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; T3E

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

CD3E

Gene ID

916

NCBI Gene ID

3003

UniProt

P07766

Swiss Prot

P07766

Host

Mouse

Species Reactivity

Human

Reactivity

CD3e

Immunogen

Human PBMCs

Target Antigen

CD3e

Clonality

Monoclonal

Clone

OKT3

Type

Primary

Source

Animal

Origin

Animal

Applications

Functional studies (published) |IHC, frozen (published) |Flow, surface (verified) |IF (verified) |IP (published)

Positive Control

Jurkat cells. Tonsil and lymph node

Stability

2 years

Concentration

1 mg/mL

Buffer

PBS, no BSA, no azide

Molecular Weight

23 kDa

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. J Cell Biol (1992) 267(17): 11685-11688. (immunoprecipitation) PNAS USA (2004) 101(20): 7675-7680. (crystal structure of antibody/antigen complex) J Immunol (2005) 174:3773-3780. (IHC, frozen) Blood (2011) 117: 5463-5472. (in vivo cell depletion) J Immunol (2012) 188:5538-5546. (functional studies)

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG2a, kappa

Species

Mus musculus (mouse)

Classification

PBS, no BSA, no sodium azide (CAS 26628-22-8)

Conjugate

Purified, BSA-free