Topoisomerase (DNA) I, Mitochondrial (TOP1MT) (TOP1MT/568), 1mg/mL

About this product

DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNUM0489-50

Size / count

1x 50 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

DNA topoisomerase I; TOP1MT; Topoisomerase I mitochondrial; Type IB Topoisomerase

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

TOP1MT

Gene ID

116447

NCBI Gene ID

528574

UniProt

Q969P6

Swiss Prot

Q969P6

Host

Mouse

Species Reactivity

Human

Reactivity

Topoisomerase I, Mitochondrial

Immunogen

Recombinant full-length human TOP1MT protein

Target Antigen

Topoisomerase I, Mitochondrial

Clonality

Monoclonal

Clone

TOP1MT/568

Type

Primary

Source

Animal

Origin

Animal

Applications

IHC, FFPE (verified)

Positive Control

A431 cells. Heart, Skeletal muscle, brain or fetal liver.

Stability

2 years

Concentration

1 mg/mL

Buffer

PBS, no BSA, no azide

Molecular Weight

70 kDa

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG1, kappa

Species

Mus musculus (mouse)

Classification

PBS, no BSA, no sodium azide (CAS 26628-22-8)

Conjugate

Purified, BSA-free