MLH1 (MutL Homolog 1) (MLH1/1324), CF640R conjugate, 0.1mg/mL

About this product

This MAb recognizes a protein of 83 kDa, identified as MLH1. Defects in MLH1 are the cause of hereditary non-polyposis colorectal cancer type 2 (HNPCC2) . Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR) . DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process, which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma, which plays a role in meiosis. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC401324-500

Size / count

1x 500 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

COCA2; DNA mismatch repair protein Mlh1; FCC2; hMLH1; MutL homolog 1 (E. coli) ; MutL homolog 1 (MLH1) ; MutL homolog 1 colon cancer nonpolyposis type 2 (HNPCC2) ; MutL homolog 1; colon cancer; nonpolyposis type 2 (E. coli) ; MutL protein homolog 1; MutL; E. coli; homolog of; 1

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

MLH1

Gene ID

4292

NCBI Gene ID

195364

UniProt

P40692

Swiss Prot

P40692

Host

Mouse

Species Reactivity

Human

Reactivity

MLH1

Immunogen

Recombinant human MLH1 protein

Target Antigen

MLH1

Clonality

Monoclonal

Clone

MLH1/1324

Type

Primary

Source

Animal

Origin

Animal

Positive Control

Molt-4, HeLa or Jurkat cells. Lymph node or Colon, breast, lung, testis, prostate or thyroid carcinoma.

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

85 kDa

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG2b, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF640R