HIF1 alpha (Hypoxia-Inducible Factor 1-alpha) (ESEE122), 0.2mg/mL

Catalog number BNUB1574-100

Price for BNUB1574-100

Size / count 1x 100 µL

About this product

HIF1 (hypoxia-inducible factor 1), a heterodimeric transcription factor complex central to cellular response to hypoxia, consists of two subunits (HIF-1 alpha and HIF-1 beta) which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family. Expression of HIF-1 alpha protein is regulated by cellular oxygen level alterations as well as in oxygen-independent manner via different cytokines (through the PI3K-AKT-mTOR pathway), growth factors, oncogenic activation, or loss of tumor suppressor function etc. In normoxic cells, HIF-1 alpha is proline hydroxylated leading to a conformational change that promotes its binding to the VLH (von Hippel Lindau) protein E3 ligase complex; ubiquitination and followed by rapid proteasomal degradation. Hypoxia as well as chemical hydroxylase inhibitors (desferrioxamine, cobalt etc.) inhibit HIF-1 alpha degradation and lead to its accumulation in the cells, whereas, contrastingly, HIF-1 beta/ARNT (AhR nuclear translocator) remains stable under both conditions. Besides their critical role in hypoxic response, HIF1s regulates the transcription of genes responsible for angiogenesis, erythropoiesis/iron-metabolism, glucose metabolism, cell proliferation/survival, adipogenesis, carotid body formation, B lymphocyte development and immune reactions. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNUB1574-100
Size / count: 1x 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78 (bHLHe78) ; Member of PAS superfamily 1 (MOP1) ; PAS domain-containing protein 8 (PASD8)
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: HIF1A
Gene ID: 3091
NCBI Gene ID: 597216
UniProt: Q16665
Swiss Prot: Q16665
Host: Mouse
Species Reactivity: Cow, Dog, Human, Mouse, Rat
Reactivity: HIF1 Alpha
Immunogen: GST-human HIF-1A amino acids 329-530 fusion protein
Target Antigen: HIF1 Alpha
Clonality: Monoclonal
Clone: ESEE122
Type: Primary
Source: Animal
Origin: Animal
Applications: IHC, FFPE (published) |WB (published)
Positive Control: Cobalt chloride treated HeLa cells. Colon, Renal, Breast or Bladder Carcinomas.
Stability: 2 years
Concentration: 0.2 mg/mL
Buffer: PBS, 0.05% BSA, 0.05% azide
Molecular Weight: 92-110 kDa
References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. Cancer (2006) 107(4): 757-766. (IHC, FFPE) Biol Pharm Bull (2017) 40(1): 82-87. (WB)
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG1, kappa
Species: Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification: PBS, 0.05% BSA, 0.05% sodium azide (CAS 26628-22-8)
Conjugate: Purified, with BSA

Frequently Asked Questions

What is the catalog number for HIF1 alpha (Hypoxia-Inducible Factor 1-alpha) (ESEE122), 0.2mg/mL?

The catalog number for HIF1 alpha (Hypoxia-Inducible Factor 1-alpha) (ESEE122), 0.2mg/mL is BNUB1574-100.

What sizes are available for HIF1 alpha (Hypoxia-Inducible Factor 1-alpha) (ESEE122), 0.2mg/mL?

HIF1 alpha (Hypoxia-Inducible Factor 1-alpha) (ESEE122), 0.2mg/mL is available in the following size(s): 1x 100 µL.

Is HIF1 alpha (Hypoxia-Inducible Factor 1-alpha) (ESEE122), 0.2mg/mL in stock?

HIF1 alpha (Hypoxia-Inducible Factor 1-alpha) (ESEE122), 0.2mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures HIF1 alpha (Hypoxia-Inducible Factor 1-alpha) (ESEE122), 0.2mg/mL?

HIF1 alpha (Hypoxia-Inducible Factor 1-alpha) (ESEE122), 0.2mg/mL is manufactured by Biotium.

What is the estimated delivery time for HIF1 alpha (Hypoxia-Inducible Factor 1-alpha) (ESEE122), 0.2mg/mL?

Estimated delivery time for HIF1 alpha (Hypoxia-Inducible Factor 1-alpha) (ESEE122), 0.2mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for HIF1 alpha (Hypoxia-Inducible Factor 1-alpha) (ESEE122), 0.2mg/mL?

The safety data sheet for HIF1 alpha (Hypoxia-Inducible Factor 1-alpha) (ESEE122), 0.2mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is HIF1 alpha (Hypoxia-Inducible Factor 1-alpha) (ESEE122), 0.2mg/mL used for?

What is the sensitivity of this ELISA kit?

Assay sensitivity, including the minimum detectable dose, is listed on the datasheet. Kits that are assembled on order typically ship within 2-4 weeks.

Do you ship this product with dry ice?

Some products, including certain cell lines and biologics, require dry ice for transportation, which carries an additional fee shown clearly in your quotation. Dry ice delivery isn’t always possible to every destination due to transit time or local restrictions, so we check feasibility in advance and you must store the goods at the correct temperature the moment they arrive.

Is this product species cross-reactive?

Cross-reactivity data, based on available testing, is listed under the reactivity section of the datasheet. Contact us if you need reactivity confirmed for a species not listed.