CD62L (L-Selectin) (LAM1-116), CF647 conjugate, 0.1mg/mL

About this product

Selectins, also designated CD62 antigens, comprise a family of carbohydrate-binding proteins involved in mediating cellular interactions with leukocytes. L-Selectin (also designated LECAM-1 or CD62L) is expressed on the majority of B and naive T cells and on most monocytes, neutrophils and eosinophils. L-Selectin interacts with specific carbohydrates expressed by activated endothelial cells. P-Selectin (also designated GMP-140 or CD62P), expressed on activated platelets and endothelial cells, and E-Selectin (also designated ELMA-1 or CD62E), expressed on endothelial cells, exhibit overlapping ligand specificities. Both recognize sialyl-Le (x) as a ligand and bind to specific carbohydrates on neutrophils and monocytes. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC471587-500

Size / count

1x 500 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

A.11; AI528707; CD62 antigen ligand; CD62L; gp90-MEL; IgA nephropathy; susceptibility to; included; L Selectin; L-selectin; LAM1; LECAM1; LEU8; Leukocyte surface antigen Leu-8; Leukocyte-endothelial cell adhesion molecule 1; Lnhr; LSEL; Ly-22; LYAM1; Lymph node homing receptor; Lymphocyte antigen 22; Lymphocyte surface MEL-14 antigen; Pln homing receptor; PLNHR; SELL; TQ1

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

SELL

Gene ID

6402

NCBI Gene ID

728756

UniProt

P14151

Swiss Prot

P14151

Host

Mouse

Species Reactivity

Human

Reactivity

CD62L|L-Selectin

Immunogen

MAb raised against supernatant from phorbol myristic acid activated human peripheral blood leukocytes.

Target Antigen

CD62L | L-Selectin

Clonality

Monoclonal

Clone

LAM1-116

Type

Primary

Source

Animal

Origin

Animal

Applications

Flow, surface (published) |Functional studies (published) |IHC, FFPE (published) |ELISA (published) |IP (published) |WB (published)

Positive Control

HPBL or Jurkat whole cell lysate.

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

65-75 kDa

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. J Clin Invest (1997) 99(8): 2036-2044. (Flow) J Clin Invest (1997) 100(5): 996-1005. (functional studies) J Biol Chem (1997) 272(21): 13961-13965. (Flow, IP) Ann Rheum Dis (2000) 59:297-299. (ELISA) J Immunol (2002) 169:2034-2043. (ELISA) Immunity (2003) 19(5): 713-724. (WB) Fert Stert (2007) 88(4) Supplement: 1207-1211. (IHC, FFPE) J Leukocyte Biol (2008) 83: 99-105. (Flow, IF)

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG1, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF647