CD22 / BL-CAM (B-Cell Marker) (RFB4), CF640R conjugate, 0.1mg/mL

Catalog number BNC401594-500

Price for BNC401594-500

Size / count 1x 500 µL

About this product

Recognizes a protein of 130-140 kDa, identified as CD22 (also known as BL-CAM) . CD22 expression is restricted to normal and neoplastic B cells and is absent from other haemopoietic cell types. In B-cell ontogeny, CD22 is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD . It is not expressed by plasma cells, CD22 is found highly expressed in follicular mantle and marginal zone B-cells, and while germinal center B-cells are relatively weak. CD22 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNC401594-500
Size / count: 1x 500 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: B-lymphocyte cell adhesion molecule (BL-CAM) ; B-cell receptor CD22; CD22; Lectin 2; Lyb8; Sialic acid-binding Ig-like lectin 2 (Siglec-2) ; SIGLEC2; T-cell surface antigen Leu-14
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: CD22
Gene ID: 933
NCBI Gene ID: 579691
UniProt: P20273
Swiss Prot: P20273
Host: Mouse
Species Reactivity: Human
Reactivity: BL-CAM|CD22
Immunogen: Human tonsil lymphocytes.
Target Antigen: BL-CAM | CD22
Clonality: Monoclonal
Clone: RFB4
Type: Primary
Source: Animal
Origin: Animal
Applications: Flow (verified) |Flow, surface (published) |Functional studies (published)
Positive Control: Ramos, Raji or Daudi cells. Human Tonsil, Lymph Node or Spleen.
Stability: 2 years
Concentration: 0.1 mg/mL
Buffer: PBS, 0.1% BSA, 0.05% azide
Molecular Weight: 130-140 kDa
References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. Clin Cancer Res (2005) 11(21): 7920-7928. (Flow, surface; functional studies) Biomolecules (2020) 10: 1140. (oncotoxins)
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Notes: Protect from light
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG1, kappa
Species: Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification: 0.05% sodium azide (CAS 26628-22-8)
Conjugate: CF640R

Frequently Asked Questions

What is the catalog number for CD22 / BL-CAM (B-Cell Marker) (RFB4), CF640R conjugate, 0.1mg/mL?

The catalog number for CD22 / BL-CAM (B-Cell Marker) (RFB4), CF640R conjugate, 0.1mg/mL is BNC401594-500.

What sizes are available for CD22 / BL-CAM (B-Cell Marker) (RFB4), CF640R conjugate, 0.1mg/mL?

CD22 / BL-CAM (B-Cell Marker) (RFB4), CF640R conjugate, 0.1mg/mL is available in the following size(s): 1x 500 µL.

Is CD22 / BL-CAM (B-Cell Marker) (RFB4), CF640R conjugate, 0.1mg/mL in stock?

CD22 / BL-CAM (B-Cell Marker) (RFB4), CF640R conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures CD22 / BL-CAM (B-Cell Marker) (RFB4), CF640R conjugate, 0.1mg/mL?

CD22 / BL-CAM (B-Cell Marker) (RFB4), CF640R conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for CD22 / BL-CAM (B-Cell Marker) (RFB4), CF640R conjugate, 0.1mg/mL?

Estimated delivery time for CD22 / BL-CAM (B-Cell Marker) (RFB4), CF640R conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for CD22 / BL-CAM (B-Cell Marker) (RFB4), CF640R conjugate, 0.1mg/mL?

The safety data sheet for CD22 / BL-CAM (B-Cell Marker) (RFB4), CF640R conjugate, 0.1mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is CD22 / BL-CAM (B-Cell Marker) (RFB4), CF640R conjugate, 0.1mg/mL used for?

What is the recommended storage temperature after reconstitution?

Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.

Can this product be used in multiplex assays?

Multiplex compatibility depends on the specific product and detection panel. Contact our technical support team with details of your multiplex system for guidance before ordering.

What happens if this product doesn’t perform as expected in my experiment?

Send us the catalogue number, product name, lot number, and a summary of your results. Our technical support team will investigate and, where needed, involve the manufacturer. This is covered under our guarantee provided the product was used before its expiry date and stored correctly.