CD22 / BL-CAM (B-Cell Marker) (RFB4), CF647 conjugate, 0.1mg/mL

Catalog number BNC471594-100

Price for BNC471594-100

Size / count 1x 100 µL

About this product

Recognizes a protein of 130-140 kDa, identified as CD22 (also known as BL-CAM) . CD22 expression is restricted to normal and neoplastic B cells and is absent from other haemopoietic cell types. In B-cell ontogeny, CD22 is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD . It is not expressed by plasma cells, CD22 is found highly expressed in follicular mantle and marginal zone B-cells, and while germinal center B-cells are relatively weak. CD22 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNC471594-100
Size / count: 1x 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: B-lymphocyte cell adhesion molecule (BL-CAM) ; B-cell receptor CD22; CD22; Lectin 2; Lyb8; Sialic acid-binding Ig-like lectin 2 (Siglec-2) ; SIGLEC2; T-cell surface antigen Leu-14
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: CD22
Gene ID: 933
NCBI Gene ID: 579691
UniProt: P20273
Swiss Prot: P20273
Host: Mouse
Species Reactivity: Human
Reactivity: BL-CAM|CD22
Immunogen: Human tonsil lymphocytes.
Target Antigen: BL-CAM | CD22
Clonality: Monoclonal
Clone: RFB4
Type: Primary
Source: Animal
Origin: Animal
Applications: Flow (verified) |Flow, surface (published) |Functional studies (published)
Positive Control: Ramos, Raji or Daudi cells. Human Tonsil, Lymph Node or Spleen.
Stability: 2 years
Concentration: 0.1 mg/mL
Buffer: PBS, 0.1% BSA, 0.05% azide
Molecular Weight: 130-140 kDa
References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. Clin Cancer Res (2005) 11(21): 7920-7928. (Flow, surface; functional studies) Biomolecules (2020) 10: 1140. (oncotoxins)
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Notes: Protect from light
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG1, kappa
Species: Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification: 0.05% sodium azide (CAS 26628-22-8)
Conjugate: CF647

Frequently Asked Questions

What is the catalog number for CD22 / BL-CAM (B-Cell Marker) (RFB4), CF647 conjugate, 0.1mg/mL?

The catalog number for CD22 / BL-CAM (B-Cell Marker) (RFB4), CF647 conjugate, 0.1mg/mL is BNC471594-100.

What sizes are available for CD22 / BL-CAM (B-Cell Marker) (RFB4), CF647 conjugate, 0.1mg/mL?

CD22 / BL-CAM (B-Cell Marker) (RFB4), CF647 conjugate, 0.1mg/mL is available in the following size(s): 1x 100 µL.

Is CD22 / BL-CAM (B-Cell Marker) (RFB4), CF647 conjugate, 0.1mg/mL in stock?

CD22 / BL-CAM (B-Cell Marker) (RFB4), CF647 conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures CD22 / BL-CAM (B-Cell Marker) (RFB4), CF647 conjugate, 0.1mg/mL?

CD22 / BL-CAM (B-Cell Marker) (RFB4), CF647 conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for CD22 / BL-CAM (B-Cell Marker) (RFB4), CF647 conjugate, 0.1mg/mL?

Estimated delivery time for CD22 / BL-CAM (B-Cell Marker) (RFB4), CF647 conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for CD22 / BL-CAM (B-Cell Marker) (RFB4), CF647 conjugate, 0.1mg/mL?

The safety data sheet for CD22 / BL-CAM (B-Cell Marker) (RFB4), CF647 conjugate, 0.1mg/mL is available here: https://cdn.gentaur.com/products/37/68dd02b1061270361e109de8/msds/bnc471594-100.pdf.

What is CD22 / BL-CAM (B-Cell Marker) (RFB4), CF647 conjugate, 0.1mg/mL used for?

How do I reconstitute this lyophilized product?

Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.

What should I check when this product first arrives?

Inspect the package immediately for transportation damage, ice loss, leakage, or breakage. Report any issues right away with photos and your order reference; our specialists will determine whether the product is still usable or needs replacing.