CD22 / BL-CAM (B-Cell Marker) (RFB4), Biotin conjugate, 0.1mg/mL

Catalog number BNCB1594-500

Price for BNCB1594-500

Size / count 1x 500 µL

About this product

Recognizes a protein of 130-140 kDa, identified as CD22 (also known as BL-CAM) . CD22 expression is restricted to normal and neoplastic B cells and is absent from other haemopoietic cell types. In B-cell ontogeny, CD22 is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD . It is not expressed by plasma cells, CD22 is found highly expressed in follicular mantle and marginal zone B-cells, and while germinal center B-cells are relatively weak. CD22 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNCB1594-500
Size / count: 1x 500 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: B-lymphocyte cell adhesion molecule (BL-CAM) ; B-cell receptor CD22; CD22; Lectin 2; Lyb8; Sialic acid-binding Ig-like lectin 2 (Siglec-2) ; SIGLEC2; T-cell surface antigen Leu-14
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: CD22
Gene ID: 933
NCBI Gene ID: 579691
UniProt: P20273
Swiss Prot: P20273
Host: Mouse
Species Reactivity: Human
Reactivity: BL-CAM|CD22
Immunogen: Human tonsil lymphocytes.
Target Antigen: BL-CAM | CD22
Clonality: Monoclonal
Clone: RFB4
Type: Primary
Source: Animal
Origin: Animal
Applications: Flow (verified) |Flow, surface (published) |Functional studies (published)
Positive Control: Ramos, Raji or Daudi cells. Human Tonsil, Lymph Node or Spleen.
Stability: 2 years
Concentration: 0.1 mg/mL
Buffer: PBS, 0.1% BSA, 0.05% azide
Molecular Weight: 130-140 kDa
References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. Clin Cancer Res (2005) 11(21): 7920-7928. (Flow, surface; functional studies) Biomolecules (2020) 10: 1140. (oncotoxins)
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG1, kappa
Species: Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification: 0.05% sodium azide (CAS 26628-22-8)
Conjugate: Biotin

Frequently Asked Questions

What is the catalog number for CD22 / BL-CAM (B-Cell Marker) (RFB4), Biotin conjugate, 0.1mg/mL?

The catalog number for CD22 / BL-CAM (B-Cell Marker) (RFB4), Biotin conjugate, 0.1mg/mL is BNCB1594-500.

What sizes are available for CD22 / BL-CAM (B-Cell Marker) (RFB4), Biotin conjugate, 0.1mg/mL?

CD22 / BL-CAM (B-Cell Marker) (RFB4), Biotin conjugate, 0.1mg/mL is available in the following size(s): 1x 500 µL.

Is CD22 / BL-CAM (B-Cell Marker) (RFB4), Biotin conjugate, 0.1mg/mL in stock?

CD22 / BL-CAM (B-Cell Marker) (RFB4), Biotin conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures CD22 / BL-CAM (B-Cell Marker) (RFB4), Biotin conjugate, 0.1mg/mL?

CD22 / BL-CAM (B-Cell Marker) (RFB4), Biotin conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for CD22 / BL-CAM (B-Cell Marker) (RFB4), Biotin conjugate, 0.1mg/mL?

Estimated delivery time for CD22 / BL-CAM (B-Cell Marker) (RFB4), Biotin conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for CD22 / BL-CAM (B-Cell Marker) (RFB4), Biotin conjugate, 0.1mg/mL?

The safety data sheet for CD22 / BL-CAM (B-Cell Marker) (RFB4), Biotin conjugate, 0.1mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is CD22 / BL-CAM (B-Cell Marker) (RFB4), Biotin conjugate, 0.1mg/mL used for?

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.

What should I check when this product first arrives?

Inspect the package immediately for transportation damage, ice loss, leakage, or breakage. Report any issues right away with photos and your order reference; our specialists will determine whether the product is still usable or needs replacing.

Does this cell line or biologic require a Material Transfer Agreement (MTA)?

Some products, including certain cell lines, plasmids, and tissue cultures, are subject to intellectual property terms and may require an MTA before we can ship. We’ll inform you in advance if this product requires one, and additional handling fees may apply.