CD99 / MIC2 (Ewing's Sarcoma Marker), 1mg/mL

About this product

Recognizes a sialoglycoprotein of 27-32 kDa, identified as CD99, or MIC2 gene product, or E2 antigen. MIC2 gene is located in the pseudo-autosomal region of the human X and Y chromosome. MIC2 gene encodes two distinct proteins, which are produced by alternative splicing of the CD99 gene transcript and are identified as bands of 30 and 32 kDa (p30/32) . Although its function is not fully understood, CD99 is implicated in various cellular processes including homotypic aggregation of T cells, upregulation of T cell receptor and MHS molecules, apoptosis of immature thymocytes and leukocyte diapedesis. CD99 is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewing's sarcoma cells and primitive peripheral neuroectodermal tumors. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNUM1646-50

Size / count

1x 50 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

12E7; E2 antigen; MIC 2X; MIC 2Y; MIC2; Protein MIC2; Surface antigen MIC2; T-cell surface glycoprotein E2

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

CD99

Gene ID

4267

NCBI Gene ID

653349

UniProt

P14209

Swiss Prot

P14209

Host

Rabbit

Species Reactivity

Human

Reactivity

CD99|MIC2

Immunogen

Recombinant human MIC2 protein

Target Antigen

CD99 | MIC2

Clonality

Polyclonal

Type

Primary

Source

Animal

Origin

Animal

Applications

IHC, FFPE (verified)

Field of Research

Cancer, Endocrinology, Immunology

Positive Control

MOLT-4 cells. Pancreas or Ewing's sarcoma.

Stability

2 years

Concentration

1 mg/mL

Buffer

PBS, no BSA, no azide

Molecular Weight

27-32 kDa

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG

Species

Oryctolagus cuniculus (domestic rabbit)

Classification

PBS, no BSA, no sodium azide (CAS 26628-22-8)

Conjugate

Purified, BSA-free