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Product details
Catalog number 12225ES10
DiscontinuedPrice for 12225ES10
Size / count 10 Tests
This variant is discontinued and cannot be added to your cart.
The Hieff Superfast DNA Methylation Bisulfite Kit (Column-based) rapidly converts unmethylated cytosines in DNA samples to uracil, while leaving methylated cytosines unchanged. During high-temperature bisulfite treatment, double-stranded DNA denatures into single strands. In the presence of HSO3-, cytosine residues undergo deamination and are converted into uracil, with methylated cytosines remaining unaltered. In subsequent PCR amplification, uracil is replaced by thymine (T). The conversion process takes only 5 minutes, accommodating DNA input ranging from 100 pg to 2 μg, and achieves a conversion efficiency of ≥99% for unmethylated cytosines. The converted DNA is suitable for downstream applications such as PCR amplification and NGS sequencing.
The catalog number for Hieff Superfast DNA Methylation Bisulfite Kit is 12225ES10.
Hieff Superfast DNA Methylation Bisulfite Kit is currently made to order / not held in immediate stock. Contact us for lead time.
The Hieff Superfast DNA Methylation Bisulfite Kit (Column-based) rapidly converts unmethylated cytosines in DNA samples to uracil, while leaving methylated cytosines unchanged. During high-temperature bisulfite treatment, double-stranded DNA denatures into single strands. In the presence of HSO3-, c
Shelf life and expiration dates vary by product and are shown on the product listing and label. Products used beyond their expiration date are not eligible for performance-related returns or refunds, so please check the lot number and expiry before use.
The expression system, such as E. coli, HEK293, or insect cells, is listed on the datasheet under production details, which can affect post-translational modifications relevant to your application.
As a consumer, you have a 14-day right of withdrawal from receipt under EU consumer protection law, submitted via our withdrawal/cancellation request form. Note this applies to unused, undamaged products, and doesn’t override the expiry/storage conditions on performance-based claims.
