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Product details

CD45RA (Leukocyte Marker) (K4B5), 0.2mg/mL

Catalog number BNUB1680-100

Price for BNUB1680-100

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Size / count 1x 100 µL

About this product

Recognizes a protein of 205 kDa-220 kDa, identified as CD45RA. CD45RA is isoforms of the human leukocyte common antigen (CD45) . Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4 T-cells, 50% of peripheral CD8 T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This MAb is useful in study on the subpopulation of CD4 or CD8 T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO ve) from B cell lymphomas (CD45RA ve) . Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number
BNUB1680-100
Size / count
1x 100 µL
Dry ice
No
Hazardous
No
Discontinued
No
CAS Number
9007-83-4
Synonyms
B220; CD45R; GP180; Leukocyte common antigen (LCA) ; Loc; Ly-5; Lyt-4; Protein tyrosine phosphatase receptor type C (PTPRC) ; Receptor-type tyrosine-protein phosphatase C; T200 glycoprotein
UNSPSC
41116161
UNSPSC Description
Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name
PTPRC
Gene ID
5788
NCBI Gene ID
654514
UniProt
P08575
Swiss Prot
P08575
Host
Mouse
Species Reactivity
Human
Reactivity
CD45RA
Immunogen
Stimulated human leukocytes
Target Antigen
CD45RA
Clonality
Monoclonal
Clone
K4B5
Type
Primary
Source
Animal
Origin
Animal
Applications
Flow (verified) |IHC, FFPE (verified) |WB (verified)
Field of Research
Immunology, Signal transduction
Positive Control
Daudi, Raji, IM9 and human lymphocytes. Human spleen, lymph nodes and tonsils.
Stability
2 years
Concentration
0.2 mg/mL
Buffer
PBS, 0.05% BSA, 0.05% azide
Molecular Weight
205-220 kDa
Shipping Conditions
RT (ND)
Storage Conditions
Stable at room temperature or 37°C (98°F) for 7 days.
Shelf Life
Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment
IgG2a, kappa
Species
Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification
PBS, 0.05% BSA, 0.05% sodium azide (CAS 26628-22-8)
Conjugate
Purified, with BSA

Frequently Asked Questions

What is the catalog number for CD45RA (Leukocyte Marker) (K4B5), 0.2mg/mL?

The catalog number for CD45RA (Leukocyte Marker) (K4B5), 0.2mg/mL is BNUB1680-100.

What sizes are available for CD45RA (Leukocyte Marker) (K4B5), 0.2mg/mL?

CD45RA (Leukocyte Marker) (K4B5), 0.2mg/mL is available in the following size(s): 1x 100 µL.

Is CD45RA (Leukocyte Marker) (K4B5), 0.2mg/mL in stock?

CD45RA (Leukocyte Marker) (K4B5), 0.2mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures CD45RA (Leukocyte Marker) (K4B5), 0.2mg/mL?

CD45RA (Leukocyte Marker) (K4B5), 0.2mg/mL is manufactured by Biotium.

What is the estimated delivery time for CD45RA (Leukocyte Marker) (K4B5), 0.2mg/mL?

Estimated delivery time for CD45RA (Leukocyte Marker) (K4B5), 0.2mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for CD45RA (Leukocyte Marker) (K4B5), 0.2mg/mL?

The safety data sheet for CD45RA (Leukocyte Marker) (K4B5), 0.2mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is CD45RA (Leukocyte Marker) (K4B5), 0.2mg/mL used for?

Recognizes a protein of 205 kDa-220 kDa, identified as CD45RA. CD45RA is isoforms of the human leukocyte common antigen (CD45) . Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms,

What is the recommended dilution for this antibody?

A recommended dilution range for each validated application is provided on the datasheet, but optimal dilution should be titrated for your specific experimental system, sample type, and detection method.

What is the recommended storage temperature after reconstitution?

Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.

Can this product be used in multiplex assays?

Multiplex compatibility depends on the specific product and detection panel. Contact our technical support team with details of your multiplex system for guidance before ordering.

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