PD1, Mouse (RMP1-14), CF647 conjugate, 0.1mg/mL

About this product

PD-1 is expressed on a subset of CD4-CD8-thymocytes, and on activated T and B cells. PD-1 is thought to be involved in lymphocyte clonal selection and peripheral tolerance. The PD-1 ligands, PD-L1 (also known as B7-H1) and PD-L2 (B7-DC), are members of the B7 immunoglobulin superfamily. PD-1 is transiently expressed on CD4 and CD8 thymocytes as well as activated T and B-lymphocytes and myeloid cells. Additionally, Pdcd1mRNA is expressed in developing B-lymphocytes during the pro-B-cell stage. The RMP1-14 antibody has been reported to block the binding of PD-1 to its ligands (B7-H1 and B7-DC) and to inhibit T cell proliferation and cytokine production co-stimulated by macrophages (but not by dendritic cells and B cells) . Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC472002-500

Size / count

1x 500 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

CD279; CD279 antigen; PD1; PDCD1; Programmed cell death 1; Programmed cell death protein 1; Systemic lupus erythematosus susceptibility 2 (SLEB2)

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene ID

18566

NCBI Gene ID

5024

UniProt

Q02242

Swiss Prot

Q02242

Host

Rat

Species Reactivity

Mouse

Reactivity

PD1

Immunogen

PDCD1 transfected BHK cells

Target Antigen

PD1

Clonality

Monoclonal

Clone

RMP1-14

Type

Primary

Source

Animal

Origin

Animal

Applications

Flow, surface (published) |Functional studies (published)

Positive Control

Spleen, Thymus or Lymph Node.

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

~50-55 kDa

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. J Immunol (2005) 175:1586-1592. (Flow; functional studies) Cancer Res (2014) 74(14): 3652-8. (functional studies) Cancer Discov (2017) 7(7): 694-703. (functional studies)

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG2a, kappa

Species

Rattus norvegicus (laboratory rat), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF647