CD8b, Mouse (H35-17.2), CF647 conjugate, 0.1mg/mL

About this product

This rat monoclonal antibody reacts with the mouse CD8 beta molecule. The CD8 beta chain associates with the CD8 alpha chain to form the CD8 alpha/beta heterodimer expressed on the surface of a majority of thymocytes, and on peripheral cytotoxic alpha beta TCR T cells. This monoclonal antibody identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC472003-100

Size / count

1x 100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

CD8b antigen; CD8 antigen beta polypeptide 1 (p37) ; CD8 beta; CD8B1; Leu2; Ly3; LYT3; P37; T lymphocyte surface glycoprotein beta chain; T-cell surface glycoprotein CD8 beta chain

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

Cd8b1 (Mouse)

Gene ID

12526

NCBI Gene ID

3331

UniProt

P10300

Swiss Prot

P10300

Host

Rat

Species Reactivity

Mouse

Reactivity

CD8b

Immunogen

Five day mixed leukocyte culture population, C57BL/6 anti-BALB/c

Target Antigen

CD8b,

Clonality

Monoclonal

Clone

H35-17.2

Type

Primary

Source

Animal

Origin

Animal

Applications

Flow, surface (published) |Functional studies (published)

Positive Control

MPBMC's or Thymus.

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

37 kDa

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. Infection Immunity (1994) 62(5): 1820-1829. (functional studies) Meth Mol Biol vol 1559. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6786-5_2 (Flow, surface)

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG2b, kappa

Species

Rattus norvegicus (laboratory rat), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF647