CD8b, Mouse (H35-17.2), Biotin conjugate, 0.1mg/mL

Catalog number BNCB2003-100

Price for BNCB2003-100

Size / count 1x 100 µL

About this product

This rat monoclonal antibody reacts with the mouse CD8 beta molecule. The CD8 beta chain associates with the CD8 alpha chain to form the CD8 alpha/beta heterodimer expressed on the surface of a majority of thymocytes, and on peripheral cytotoxic alpha beta TCR T cells. This monoclonal antibody identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNCB2003-100
Size / count: 1x 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: CD8b antigen; CD8 antigen beta polypeptide 1 (p37) ; CD8 beta; CD8B1; Leu2; Ly3; LYT3; P37; T lymphocyte surface glycoprotein beta chain; T-cell surface glycoprotein CD8 beta chain
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: Cd8b1 (Mouse)
Gene ID: 12526
NCBI Gene ID: 3331
UniProt: P10300
Swiss Prot: P10300
Host: Rat
Species Reactivity: Mouse
Reactivity: CD8b
Immunogen: Five day mixed leukocyte culture population, C57BL/6 anti-BALB/c
Target Antigen: CD8b,
Clonality: Monoclonal
Clone: H35-17.2
Type: Primary
Source: Animal
Origin: Animal
Applications: Flow, surface (published) |Functional studies (published)
Positive Control: MPBMC's or Thymus.
Stability: 2 years
Concentration: 0.1 mg/mL
Buffer: PBS, 0.1% BSA, 0.05% azide
Molecular Weight: 37 kDa
References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. Infection Immunity (1994) 62(5): 1820-1829. (functional studies) Meth Mol Biol vol 1559. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6786-5_2 (Flow, surface)
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG2b, kappa
Species: Rattus norvegicus (laboratory rat), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification: 0.05% sodium azide (CAS 26628-22-8)
Conjugate: Biotin

Frequently Asked Questions

What is the catalog number for CD8b, Mouse (H35-17.2), Biotin conjugate, 0.1mg/mL?

The catalog number for CD8b, Mouse (H35-17.2), Biotin conjugate, 0.1mg/mL is BNCB2003-100.

What sizes are available for CD8b, Mouse (H35-17.2), Biotin conjugate, 0.1mg/mL?

CD8b, Mouse (H35-17.2), Biotin conjugate, 0.1mg/mL is available in the following size(s): 1x 100 µL.

Is CD8b, Mouse (H35-17.2), Biotin conjugate, 0.1mg/mL in stock?

CD8b, Mouse (H35-17.2), Biotin conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures CD8b, Mouse (H35-17.2), Biotin conjugate, 0.1mg/mL?

CD8b, Mouse (H35-17.2), Biotin conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for CD8b, Mouse (H35-17.2), Biotin conjugate, 0.1mg/mL?

Estimated delivery time for CD8b, Mouse (H35-17.2), Biotin conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for CD8b, Mouse (H35-17.2), Biotin conjugate, 0.1mg/mL?

The safety data sheet for CD8b, Mouse (H35-17.2), Biotin conjugate, 0.1mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is CD8b, Mouse (H35-17.2), Biotin conjugate, 0.1mg/mL used for?

How long will it take to receive this product if it’s custom made?

Lead times depend on the product type: custom polyclonal antibodies typically take 12-14 weeks, monoclonal antibodies 15-17 weeks, made-to-order recombinant proteins 4-8 weeks, and made-to-order recombinant antibodies 6-8 weeks. These are estimates and may shift due to production delays.

What is the recommended dilution for this antibody?

A recommended dilution range for each validated application is provided on the datasheet, but optimal dilution should be titrated for your specific experimental system, sample type, and detection method.

What is the recommended storage temperature after reconstitution?

Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.