CD45 / LCA (Leucocyte Marker) (PTPRC/1147 + PTPRC/1460), CF594 conjugate, 0.1mg/mL

Catalog number BNC942027-500

Price for BNC942027-500

Size / count 1x 500 µL

About this product

Recognizes the CD45 leukocyte common antigen (LCA) family which is comprised of at least four isoforms of membrane glycoproteins (220, 205, 190, 180 kDa) expressed on hematopoietic cell lines but absent on non-hematopoietic cell lines, normal and malignant non-hematopoietic tissues. The intracellular portions of these molecules have protein phosphatase activity and are involved in regulation of transmembrane signals. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms. A positive result with this MAb is highly indicative of lymphoid or myeloid origin. Certain types of lymphoid neoplasms may lack CD45 (Hodgkin lymphoma, some T-cell lymphomas, and some leukemias) so its absence does not rule out a hematolymphoid tumor. This antibody is expressed almost exclusively by cells of hematopoietic lineage and is present in most benign and malignant lymphocytes as well as plasma cell precursors. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNC942027-500
Size / count: 1x 500 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: B220; CD45R; GP180; Leukocyte common antigen (LCA) ; Loc; Ly-5; Lyt-4; Protein tyrosine phosphatase receptor type C (PTPRC) ; Receptor-type tyrosine-protein phosphatase C; T200 glycoprotein
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: PTPRC
Gene ID: 5788
NCBI Gene ID: 654514
UniProt: P08575
Swiss Prot: P08575
Host: Mouse
Species Reactivity: Human
Reactivity: CD45|LCA
Immunogen: Human recombinant full-length CD45 protein
Target Antigen: CD45 | LCA
Clonality: Monoclonal
Clone: PTPRC/1147 PTPRC/1460
Type: Primary
Source: Animal
Origin: Animal
Applications: IHC, FFPE (verified) |WB (verified)
Field of Research: Immunology, Signal transduction
Positive Control: Ramos, U-698, or GA-10 cells. Tonsil
Stability: 2 years
Concentration: 0.1 mg/mL
Buffer: PBS, 0.1% BSA, 0.05% azide
Molecular Weight: 180-220 kDa
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Notes: Protect from light
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG1, kappa
Species: Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification: 0.05% sodium azide (CAS 26628-22-8)
Conjugate: CF594

Frequently Asked Questions

What is the catalog number for CD45 / LCA (Leucocyte Marker) (PTPRC/1147 + PTPRC/1460), CF594 conjugate, 0.1mg/mL?

The catalog number for CD45 / LCA (Leucocyte Marker) (PTPRC/1147 + PTPRC/1460), CF594 conjugate, 0.1mg/mL is BNC942027-500.

What sizes are available for CD45 / LCA (Leucocyte Marker) (PTPRC/1147 + PTPRC/1460), CF594 conjugate, 0.1mg/mL?

CD45 / LCA (Leucocyte Marker) (PTPRC/1147 + PTPRC/1460), CF594 conjugate, 0.1mg/mL is available in the following size(s): 1x 500 µL.

Is CD45 / LCA (Leucocyte Marker) (PTPRC/1147 + PTPRC/1460), CF594 conjugate, 0.1mg/mL in stock?

CD45 / LCA (Leucocyte Marker) (PTPRC/1147 + PTPRC/1460), CF594 conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures CD45 / LCA (Leucocyte Marker) (PTPRC/1147 + PTPRC/1460), CF594 conjugate, 0.1mg/mL?

CD45 / LCA (Leucocyte Marker) (PTPRC/1147 + PTPRC/1460), CF594 conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for CD45 / LCA (Leucocyte Marker) (PTPRC/1147 + PTPRC/1460), CF594 conjugate, 0.1mg/mL?

Estimated delivery time for CD45 / LCA (Leucocyte Marker) (PTPRC/1147 + PTPRC/1460), CF594 conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for CD45 / LCA (Leucocyte Marker) (PTPRC/1147 + PTPRC/1460), CF594 conjugate, 0.1mg/mL?

The safety data sheet for CD45 / LCA (Leucocyte Marker) (PTPRC/1147 + PTPRC/1460), CF594 conjugate, 0.1mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is CD45 / LCA (Leucocyte Marker) (PTPRC/1147 + PTPRC/1460), CF594 conjugate, 0.1mg/mL used for?

Is this kit compatible with automated liquid handling platforms?

Automation compatibility varies by kit and isn’t always listed on the datasheet. Contact our technical support team with details of your platform so we can confirm with the manufacturer.

What applications has this product been validated for?

Validated applications such as WB, ELISA, IHC, ICC, or FC are listed on the product datasheet. If you need application-specific validation data before ordering, contact our technical support team with the catalogue number.

How do I reconstitute this lyophilized product?

Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.