ARF1 (Golgi Apparatus Marker) (3F1), 0.2mg/mL

About this product

The ADP-ribosylation factor (ARF) family comprises a group of structurally and functionally conserved proteins, which are members of the Ras superfamily of regulatory GTP-binding proteins. The ARF family is divided functionally into the ARF and the ARF-like proteins. ARF's share more than 60% sequence identity, appear to be ubiquitous in eukaryotes, and are highly conserved evolutionarily. ARF is involved in intracellular protein traffic to and within the Golgi complex. ARF has a number of disparate activities including maintenance of organelle integrity, assembly of coat proteins, as a co-factor for cholera toxin and as an activator of phospholipase D. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNUB2029-100

Size / count

1x 100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

ADP-ribosylation factor 1; ARF1

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

ARF1

Gene ID

375

NCBI Gene ID

286221

UniProt

P84077

Swiss Prot

P84077

Host

Mouse

Species Reactivity

Human, Mouse, Rat

Reactivity

ADP-Ribosylation Factor 1|ARF1

Immunogen

Synthetic peptide SNQLRNQ, corresponding to C terminal amino acids 174-180 of Human ARF1.

Target Antigen

ADP-Ribosylation Factor 1 | ARF1

Clonality

Monoclonal

Clone

3F1

Type

Primary

Source

Animal

Origin

Animal

Applications

IF (published) |WB (published)

Positive Control

HeLa cells. Kidney, Liver, Brain or Breast.

Stability

2 years

Concentration

0.2 mg/mL

Buffer

PBS, 0.05% BSA, 0.05% azide

Molecular Weight

20 kDa

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. J Biol Chem (2009) 284(20): 13948-13957. (WB) J Biochem (2016) 159(2): 201-208. (IF; WB)

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG2a, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

PBS, 0.05% BSA, 0.05% sodium azide (CAS 26628-22-8)

Conjugate

Purified, with BSA