Gp100 / Melanosome / PMEL17 / SILV (Melanoma Marker) (PMEL/2038), 1mg/mL

About this product

Cytotoxic T lymphocytes (CTLs) recognize melanoma-associated antigens, which belong to three main groups. These groups include tumor-associated testis-specific antigens, melanocyte differentiation antigens and mutated or aberrantly expressed antigens, which are routinely used as markers to identify melanomas based on their binding to specific monoclonal antibodies. gp100, also designated ME20-M, ME20-S and PMEL 17, is classified as a melanocyte differentiation antigen and is expressed at low levels in normal cell lines and tissues, but is upregulated in melanocytes. gp100 is a highly glycosylated protein. It is also the product of proteolytic cleavage, which results in a secreted protein.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNUM2038-50

Size / count

1x 50 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

95kDa melanocyte-specific secreted glycoprotein, M-beta; Melanocyte lineage specific antigen GP100; Melanocyte protein Pmel 17; Melanoma associated ME20 antigen; Melanosomal matrix protein17; Premelanosome protein (PMEL17) ; Secreted melanoma-associated ME20 antigen; SILV; Silver homolog

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

SILV

Gene ID

6490

NCBI Gene ID

95972

UniProt

P40967

Swiss Prot

P40967

Host

Mouse

Species Reactivity

Human

Reactivity

Gp100|Melanosome|Pmel17|SILV

Immunogen

Recombinant human SILV protein fragment (around aa 376-502) (exact sequence is proprietary)

Target Antigen

gp100 | Melanosome | Pmel17 | SILV

Clonality

Monoclonal

Clone

PMEL/2038

Type

Primary

Source

Animal

Origin

Animal

Applications

IHC, FFPE (verified) |WB (verified)

Field of Research

Cancer

Positive Control

SK-MEL-28 cells or Melanoma

Stability

2 years

Concentration

1 mg/mL

Buffer

PBS, no BSA, no azide

Molecular Weight

90-100 kDa

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG1, kappa

Species

Mus musculus (mouse)

Classification

PBS, no BSA, no sodium azide (CAS 26628-22-8)

Conjugate

Purified, BSA-free