DNA Ligase 1 (1A9), Biotin conjugate, 0.1mg/mL

Catalog number BNCB2177-100

Price for BNCB2177-100

Size / count 1x 100 µL

About this product

DNA Ligase I maintains the major DNA Ligase activity in proliferating cells by joining Okazaki fragments during lagging strand DNA replication. Human DNA Ligase I also has an essential role in DNA repair pathways, where it catalyzes the formation of phosphodiester bonds between adjacent 5' phosphoryl and 3' hydroxy termini at single breaks in duplex DNA molecules. In addition, DNA Ligase I plays a role in sealing nicks during excision repair. Similar to other DNA ligases, DNA Ligase I is built around a common catalytic core. Increased levels of DNA Ligase I are found in human tumors, as compared to benign tissues, as well as in peripheral blood lymphocytes. DNA Ligase I activity is altered in the chromosomal breakage deficit Bloom's syndrome (BS) . Individuals with BS either have decreased levels of abnormally thermolabile DNA Ligase I or possess a dimeric form of this enzyme. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNCB2177-100
Size / count: 1x 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: DNA ligase 1; DNA ligase I; DNLI1_HUMAN; LIG 1; lig1; Ligase I DNA ATP dependent; MGC117397; MGC130025; Polydeoxyribonucleotide synthase [ATP] 1
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: LIG1
Gene ID: 3978
NCBI Gene ID: 1770
UniProt: P18858
Swiss Prot: P18858
Host: Mouse
Species Reactivity: Bovine, Human, Mouse, Rat
Reactivity: DNA Ligase 1
Immunogen: Full-length native cow LIG1 protein
Target Antigen: DNA Ligase 1
Clonality: Monoclonal
Clone: 1A9
Type: Primary
Source: Animal
Origin: Animal
Applications: Flow, surface (published) |Functional studies (published) |IP (published)
Positive Control: MOLT-4, PC-3 or Jurkat cell lysates.
Stability: 2 years
Concentration: 0.1 mg/mL
Buffer: PBS, 0.1% BSA, 0.05% azide
Molecular Weight: 133 kDa
References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. PNAS USA (1987) 84:1374-1378. (Flow, IP) Eur J Immunol (1990) 20: 509-515. (functional studies) J Immunol (2003) 171:2496-2503. (Flow; IP; functional studies)
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG1, kappa
Species: Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification: 0.05% sodium azide (CAS 26628-22-8)
Conjugate: Biotin

Frequently Asked Questions

What is the catalog number for DNA Ligase 1 (1A9), Biotin conjugate, 0.1mg/mL?

The catalog number for DNA Ligase 1 (1A9), Biotin conjugate, 0.1mg/mL is BNCB2177-100.

What sizes are available for DNA Ligase 1 (1A9), Biotin conjugate, 0.1mg/mL?

DNA Ligase 1 (1A9), Biotin conjugate, 0.1mg/mL is available in the following size(s): 1x 100 µL.

Is DNA Ligase 1 (1A9), Biotin conjugate, 0.1mg/mL in stock?

DNA Ligase 1 (1A9), Biotin conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures DNA Ligase 1 (1A9), Biotin conjugate, 0.1mg/mL?

DNA Ligase 1 (1A9), Biotin conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for DNA Ligase 1 (1A9), Biotin conjugate, 0.1mg/mL?

Estimated delivery time for DNA Ligase 1 (1A9), Biotin conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for DNA Ligase 1 (1A9), Biotin conjugate, 0.1mg/mL?

The safety data sheet for DNA Ligase 1 (1A9), Biotin conjugate, 0.1mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is DNA Ligase 1 (1A9), Biotin conjugate, 0.1mg/mL used for?

How do I reconstitute this lyophilized product?

Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.

What should I check when this product first arrives?

Inspect the package immediately for transportation damage, ice loss, leakage, or breakage. Report any issues right away with photos and your order reference; our specialists will determine whether the product is still usable or needs replacing.