EGFR (Epidermal Growth Factor Receptor) (F4), Biotin conjugate, 0.1mg/mL

Catalog number BNCB2180-100

Price for BNCB2180-100

Size / count 1x 100 µL

About this product

This MAb recognizes a protein of 170 kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNCB2180-100
Size / count: 1x 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: ERBB1; HER1; mENA; PIG61; Proto-oncogene c-ErbB-1; Receptor Tyrosine Protein Kinase; ErbB1; Urogastrone
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: EGFR
Gene ID: 1956
NCBI Gene ID: 488293
UniProt: P00533
Swiss Prot: P00533
Host: Mouse
Species Reactivity: Human
Reactivity: EGFR
Immunogen: A synthetic peptide corresponding to aa 985-996 of human EGFR
Target Antigen: EGFR
Clonality: Monoclonal
Clone: F4
Type: Primary
Source: Animal
Origin: Animal
Applications: WB (verified)
Positive Control: A431 cells. Placenta, Breast, Colon or Bladder Carcinoma
Stability: 2 years
Concentration: 0.1 mg/mL
Buffer: PBS, 0.1% BSA, 0.05% azide
Molecular Weight: ~170 kDa (wild type) and ~145 kDa (vIII variant)
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG1, kappa
Species: Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification: 0.05% sodium azide (CAS 26628-22-8)
Conjugate: Biotin

Frequently Asked Questions

What is the catalog number for EGFR (Epidermal Growth Factor Receptor) (F4), Biotin conjugate, 0.1mg/mL?

The catalog number for EGFR (Epidermal Growth Factor Receptor) (F4), Biotin conjugate, 0.1mg/mL is BNCB2180-100.

What sizes are available for EGFR (Epidermal Growth Factor Receptor) (F4), Biotin conjugate, 0.1mg/mL?

EGFR (Epidermal Growth Factor Receptor) (F4), Biotin conjugate, 0.1mg/mL is available in the following size(s): 1x 100 µL.

Is EGFR (Epidermal Growth Factor Receptor) (F4), Biotin conjugate, 0.1mg/mL in stock?

EGFR (Epidermal Growth Factor Receptor) (F4), Biotin conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures EGFR (Epidermal Growth Factor Receptor) (F4), Biotin conjugate, 0.1mg/mL?

EGFR (Epidermal Growth Factor Receptor) (F4), Biotin conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for EGFR (Epidermal Growth Factor Receptor) (F4), Biotin conjugate, 0.1mg/mL?

Estimated delivery time for EGFR (Epidermal Growth Factor Receptor) (F4), Biotin conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for EGFR (Epidermal Growth Factor Receptor) (F4), Biotin conjugate, 0.1mg/mL?

The safety data sheet for EGFR (Epidermal Growth Factor Receptor) (F4), Biotin conjugate, 0.1mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is EGFR (Epidermal Growth Factor Receptor) (F4), Biotin conjugate, 0.1mg/mL used for?

What is the molecular weight of the target protein?

Molecular weight information, where applicable, is listed on the datasheet under target details, useful for confirming the correct band on western blot.

How long will it take to receive this product if it’s custom made?

Lead times depend on the product type: custom polyclonal antibodies typically take 12-14 weeks, monoclonal antibodies 15-17 weeks, made-to-order recombinant proteins 4-8 weeks, and made-to-order recombinant antibodies 6-8 weeks. These are estimates and may shift due to production delays.

What is the recommended dilution for this antibody?

A recommended dilution range for each validated application is provided on the datasheet, but optimal dilution should be titrated for your specific experimental system, sample type, and detection method.