Occludin (Marker of Early Blood Brain Barrier Damage) (OCLN/2181), CF647 conjugate, 0.1mg/mL

Catalog number BNC472181-500

Price for BNC472181-500

Size / count 1x 500 µL

About this product

Occludin is a tetraspan integral membrane protein in epithelial and endothelial tight junction (TJ) structures that can contain two extracellular loops. The protein exists in a variety of phosphorylated forms. Phosphorylation is involved in regulating both the localization and the function of Occludin. Expression of Occludin is upregulated by polyunsaturated fatty acids, increasing trans-endothelial cell resistance and reducing cellular permeability to large molecules. The level of Occludin varies greatly depending on tissue; in brain tissue, Occludin is highly expressed at cell-cell contact sites. Non-neural tissues show lower expression and discontinuous distribution. Occludin is a tight junction protein that is a key structural component of the blood-brain barrier (BBB) . Degradation of Occludin is frequently seen in ischemic stroke and contributes to BBB disruption. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNC472181-500
Size / count: 1x 500 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: BLCPMG; Occludin; OCLN; Phosphatase 1 regulatory subunit 115; PTORCH1; Tight junction protein occludin
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: OCLN
Gene ID: 100506658
NCBI Gene ID: 592605
UniProt: Q16625
Swiss Prot: Q16625
Host: Mouse
Species Reactivity: Human
Reactivity: Occludin
Immunogen: Recombinant fragment of human Occludin protein (around aa 282-415) (exact sequence is proprietary)
Target Antigen: Occludin
Clonality: Monoclonal
Clone: OCLN/2181
Type: Primary
Source: Animal
Origin: Animal
Applications: IHC, FFPE (verified) |WB (verified)
Positive Control: HepG2 cells. Kidney.
Stability: 2 years
Concentration: 0.1 mg/mL
Buffer: PBS, 0.1% BSA, 0.05% azide
Molecular Weight: 60-82 kDa
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Notes: Protect from light
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG1, kappa
Species: Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification: 0.05% sodium azide (CAS 26628-22-8)
Conjugate: CF647

Frequently Asked Questions

What is the catalog number for Occludin (Marker of Early Blood Brain Barrier Damage) (OCLN/2181), CF647 conjugate, 0.1mg/mL?

The catalog number for Occludin (Marker of Early Blood Brain Barrier Damage) (OCLN/2181), CF647 conjugate, 0.1mg/mL is BNC472181-500.

What sizes are available for Occludin (Marker of Early Blood Brain Barrier Damage) (OCLN/2181), CF647 conjugate, 0.1mg/mL?

Occludin (Marker of Early Blood Brain Barrier Damage) (OCLN/2181), CF647 conjugate, 0.1mg/mL is available in the following size(s): 1x 500 µL.

Is Occludin (Marker of Early Blood Brain Barrier Damage) (OCLN/2181), CF647 conjugate, 0.1mg/mL in stock?

Occludin (Marker of Early Blood Brain Barrier Damage) (OCLN/2181), CF647 conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Occludin (Marker of Early Blood Brain Barrier Damage) (OCLN/2181), CF647 conjugate, 0.1mg/mL?

Occludin (Marker of Early Blood Brain Barrier Damage) (OCLN/2181), CF647 conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for Occludin (Marker of Early Blood Brain Barrier Damage) (OCLN/2181), CF647 conjugate, 0.1mg/mL?

Estimated delivery time for Occludin (Marker of Early Blood Brain Barrier Damage) (OCLN/2181), CF647 conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Occludin (Marker of Early Blood Brain Barrier Damage) (OCLN/2181), CF647 conjugate, 0.1mg/mL?

The safety data sheet for Occludin (Marker of Early Blood Brain Barrier Damage) (OCLN/2181), CF647 conjugate, 0.1mg/mL is available here: https://cdn.gentaur.com/products/37/68dd02a7061270361e10641d/msds/bnc472181-500.pdf.

What is Occludin (Marker of Early Blood Brain Barrier Damage) (OCLN/2181), CF647 conjugate, 0.1mg/mL used for?

How long will it take to receive this product if it’s custom made?

Lead times depend on the product type: custom polyclonal antibodies typically take 12-14 weeks, monoclonal antibodies 15-17 weeks, made-to-order recombinant proteins 4-8 weeks, and made-to-order recombinant antibodies 6-8 weeks. These are estimates and may shift due to production delays.

What is the recommended dilution for this antibody?

A recommended dilution range for each validated application is provided on the datasheet, but optimal dilution should be titrated for your specific experimental system, sample type, and detection method.

What is the recommended storage temperature after reconstitution?

Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.