Occludin (Marker of Early Blood Brain Barrier Damage) (OCLN/2181), 0.2mg/mL

Catalog number BNUB2181-500

Price for BNUB2181-500

Size / count 1x 500 µL

About this product

Occludin is a tetraspan integral membrane protein in epithelial and endothelial tight junction (TJ) structures that can contain two extracellular loops. The protein exists in a variety of phosphorylated forms. Phosphorylation is involved in regulating both the localization and the function of Occludin. Expression of Occludin is upregulated by polyunsaturated fatty acids, increasing trans-endothelial cell resistance and reducing cellular permeability to large molecules. The level of Occludin varies greatly depending on tissue; in brain tissue, Occludin is highly expressed at cell-cell contact sites. Non-neural tissues show lower expression and discontinuous distribution. Occludin is a tight junction protein that is a key structural component of the blood-brain barrier (BBB) . Degradation of Occludin is frequently seen in ischemic stroke and contributes to BBB disruption. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNUB2181-500
Size / count: 1x 500 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: BLCPMG; Occludin; OCLN; Phosphatase 1 regulatory subunit 115; PTORCH1; Tight junction protein occludin
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: OCLN
Gene ID: 100506658
NCBI Gene ID: 592605
UniProt: Q16625
Swiss Prot: Q16625
Host: Mouse
Species Reactivity: Human
Reactivity: Occludin
Immunogen: Recombinant fragment of human Occludin protein (around aa 282-415) (exact sequence is proprietary)
Target Antigen: Occludin
Clonality: Monoclonal
Clone: OCLN/2181
Type: Primary
Source: Animal
Origin: Animal
Applications: IHC, FFPE (verified) |WB (verified)
Positive Control: HepG2 cells. Kidney.
Stability: 2 years
Concentration: 0.2 mg/mL
Buffer: PBS, 0.05% BSA, 0.05% azide
Molecular Weight: 60-82 kDa
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG1, kappa
Species: Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification: PBS, 0.05% BSA, 0.05% sodium azide (CAS 26628-22-8)
Conjugate: Purified, with BSA

Frequently Asked Questions

What is the catalog number for Occludin (Marker of Early Blood Brain Barrier Damage) (OCLN/2181), 0.2mg/mL?

The catalog number for Occludin (Marker of Early Blood Brain Barrier Damage) (OCLN/2181), 0.2mg/mL is BNUB2181-500.

What sizes are available for Occludin (Marker of Early Blood Brain Barrier Damage) (OCLN/2181), 0.2mg/mL?

Occludin (Marker of Early Blood Brain Barrier Damage) (OCLN/2181), 0.2mg/mL is available in the following size(s): 1x 500 µL.

Is Occludin (Marker of Early Blood Brain Barrier Damage) (OCLN/2181), 0.2mg/mL in stock?

Occludin (Marker of Early Blood Brain Barrier Damage) (OCLN/2181), 0.2mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Occludin (Marker of Early Blood Brain Barrier Damage) (OCLN/2181), 0.2mg/mL?

Occludin (Marker of Early Blood Brain Barrier Damage) (OCLN/2181), 0.2mg/mL is manufactured by Biotium.

What is the estimated delivery time for Occludin (Marker of Early Blood Brain Barrier Damage) (OCLN/2181), 0.2mg/mL?

Estimated delivery time for Occludin (Marker of Early Blood Brain Barrier Damage) (OCLN/2181), 0.2mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Occludin (Marker of Early Blood Brain Barrier Damage) (OCLN/2181), 0.2mg/mL?

The safety data sheet for Occludin (Marker of Early Blood Brain Barrier Damage) (OCLN/2181), 0.2mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is Occludin (Marker of Early Blood Brain Barrier Damage) (OCLN/2181), 0.2mg/mL used for?

What applications has this product been validated for?

Validated applications such as WB, ELISA, IHC, ICC, or FC are listed on the product datasheet. If you need application-specific validation data before ordering, contact our technical support team with the catalogue number.

How do I reconstitute this lyophilized product?

Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.