HLA-DR (MHC II) (TAL 1B5), CF488A conjugate, 0.1mg/mL

Catalog number BNC882187-100

Price for BNC882187-100

Size / count 1x 100 µL

About this product

Major histocompatibility complex (MHC) class II molecules destined for presentation to CD4 helper T cells is determined by two key events. These events include the dissociation of class II-associated invariant chain peptides (CLIP) from an antigen binding groove in MHC class II/ dimers through the activity of MHC molecules HLA-DM and -DO, and subsequent peptide antigen binding. Accumulating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM, -DO molecules regulate the dissociation of CLIP and the sub- sequent binding of exogenous peptides to HLA class II molecules (HLA-DR, -DQ and -DP) by sustaining a conformation that favors peptide exchange. RFLP analysis of HLA-DM genes from rheumatoid arthritis (RA) patients suggests that certain polymorphisms are genetic factors for RA susceptibility. HLA-B belongs to the HLA class I heavy chain paralogs. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. HLA-B and -C can form heterodimers consisting of a membrane anchored heavy chain and a light chain (-Microglobulin) . Polymorphisms yield hundreds of HLA-B and -C alleles. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNC882187-100
Size / count: 1x 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: DR alpha chain; DRB1; DRB4; HLA class II histocompatibility antigen DR alpha chain; HLA DR1B; HLA DR3B; HLA DRA; HLA-DRA; HLADR4B; HLADRA1; HLADRB; Major histocompatibility complex class II DR alpha; Major histocompatibility complex class II DR beta 1/3/4/5
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: HLA-DR
Gene ID: 3122
NCBI Gene ID: 520048
UniProt: P01903
Swiss Prot: P01903
Host: Mouse
Species Reactivity: Human
Reactivity: HLA-DR
Target Antigen: HLA-DR
Clonality: Monoclonal
Clone: TAL 1B5
Type: Primary
Source: Animal
Origin: Animal
Applications: Flow (verified) |IHC, FFPE (verified) |WB (verified)
Positive Control: Raji, Ramos or Daudi whole cell lysates. Spleen, tonsil or liver tissue lysates. Human melanoma, tonsil or lymph node.
Stability: 2 years
Concentration: 0.1 mg/mL
Buffer: PBS, 0.1% BSA, 0.05% azide
Molecular Weight: 36 kDa (alpha chain) ; 27 kDa (beta chain) ; 63 kDa (HLA-DR dimer)
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Notes: Protect from light
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG1, kappa
Species: Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification: 0.05% sodium azide (CAS 26628-22-8)
Conjugate: CF488A

Frequently Asked Questions

What is the catalog number for HLA-DR (MHC II) (TAL 1B5), CF488A conjugate, 0.1mg/mL?

The catalog number for HLA-DR (MHC II) (TAL 1B5), CF488A conjugate, 0.1mg/mL is BNC882187-100.

What sizes are available for HLA-DR (MHC II) (TAL 1B5), CF488A conjugate, 0.1mg/mL?

HLA-DR (MHC II) (TAL 1B5), CF488A conjugate, 0.1mg/mL is available in the following size(s): 1x 100 µL.

Is HLA-DR (MHC II) (TAL 1B5), CF488A conjugate, 0.1mg/mL in stock?

HLA-DR (MHC II) (TAL 1B5), CF488A conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures HLA-DR (MHC II) (TAL 1B5), CF488A conjugate, 0.1mg/mL?

HLA-DR (MHC II) (TAL 1B5), CF488A conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for HLA-DR (MHC II) (TAL 1B5), CF488A conjugate, 0.1mg/mL?

Estimated delivery time for HLA-DR (MHC II) (TAL 1B5), CF488A conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for HLA-DR (MHC II) (TAL 1B5), CF488A conjugate, 0.1mg/mL?

The safety data sheet for HLA-DR (MHC II) (TAL 1B5), CF488A conjugate, 0.1mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is HLA-DR (MHC II) (TAL 1B5), CF488A conjugate, 0.1mg/mL used for?

What applications has this product been validated for?

Validated applications such as WB, ELISA, IHC, ICC, or FC are listed on the product datasheet. If you need application-specific validation data before ordering, contact our technical support team with the catalogue number.

How do I reconstitute this lyophilized product?

Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.