CD50 / ICAM3 (CG106), 1mg/mL

About this product

Recognizes the D1 domain of an N-glycosylated glycoprotein of 120 kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (Leucocyte Workshop VI) . CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This MAb is excellent for staining of formalin/paraffin tissues. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNUM2216-50

Size / count

1x 50 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

CD50; CDW 50; CDw50; ICAM 3; ICAM-3; ICAM-R; ICAM3; ICAM3_HUMAN; ICAMR; Intercellular adhesion molecule 3

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

ICAM3

Gene ID

3385

NCBI Gene ID

654563

UniProt

P32942

Swiss Prot

P32942

Host

Mouse

Species Reactivity

Human

Reactivity

CD50|ICAM3

Immunogen

Stimulated human leukocytes

Target Antigen

CD50 | ICAM3

Clonality

Monoclonal

Clone

CG106

Type

Primary

Source

Animal

Origin

Animal

Applications

Flow, surface (published) |Functional studies (published) |ELISA (published)

Field of Research

Immunology

Positive Control

HL-60 or THP-1 cells. Lymph node and tonsil

Stability

2 years

Concentration

1 mg/mL

Buffer

PBS, no BSA, no azide

Molecular Weight

110-160 kDa

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. J Biol Chem (1995) 270(2): 877-884. (ELISA) J Invest Dermatol (1995) 105, 215-219.(functional studies) J Cell Biol (1995) 129(4):1143-1153. (Flow, surface) J Immunol 1998; 161:1363-1370. (ELISA; epitope mapping) Am J Pathol (2001) 159(1): 285-295. (Flow, surface)

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG2a, kappa

Species

Mus musculus (mouse)

Classification

PBS, no BSA, no sodium azide (CAS 26628-22-8)

Conjugate

Purified, BSA-free