Geminin / DNA Replication Inhibitor (CPTC-GMMN-1), Biotin conjugate, 0.1mg/mL

About this product

Geminin is a nuclear protein that regulates the initiation of DNA replication during the cell cycle. DNA replication requires the coordinated association of Cdc6 and minichromosome maintenance (MCM) proteins with chromatin. Geminin blocks this assembly of the MCM into the prereplication complex and, in turn, prevents replication from occurring. Expression of Geminin fluctuates throughout the cell cycle with Geminin levels lowest at G1. Throughout S, G2 and M phases, Geminin levels are consistently elevated followed by a decrease during mitosis. The initiation of DNA replication is dependent on the degradation of Geminin during mitosis and the absence of Geminin throughout G1 phase. Geminin degradation is mediated by the anaphasepromoting complex (APC), which specifically targets B-type cyclins and other proteins containing a destruction box motif for degradation by ubiquitinmediated proteolysis. While geminin expression is essential in maintaining chromosomal integrity, it is frequently overexpressed in cancers and evidence suggests that it plays a significant role in tumor proliferation and progression. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNCB2240-100

Size / count

1x 100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

DNA replication inhibitor; Gem; Geminin DNA replication inhibitor; GMMN; RP3 369A17.3

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

GMMN

Gene ID

51053

NCBI Gene ID

234896

UniProt

O75496

Swiss Prot

O75496

Host

Mouse

Species Reactivity

Human

Reactivity

DNA Replication Inhibitor|Geminin

Immunogen

Recombinant human full-length protein

Target Antigen

DNA Replication Inhibitor | Geminin

Clonality

Monoclonal

Clone

CPTC-GMMN-1

Type

Primary

Source

Animal

Origin

Animal

Applications

IF (verified)

Positive Control

HEK293, HeLa, MCF-7, Tonsil, Triple-negative breast cancer.

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

35 kDa

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG1, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

Biotin