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Product details

Heat Shock 27kDa Protein 1 (HPS27) (CPTC-HSPB1-2), Biotin conjugate, 0.1mg/mL

Catalog number BNCB2244-100

Price for BNCB2244-100

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Size / count 1x 100 µL

About this product

HSP27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins produced by all organisms studied. HSP27 synthesis is induced by elevated temperature, as well as by estrogen in hormone responsive cells. Interestingly, human HSP27 also shares greater than 50% homology with low molecular weight Drosophila HSPs and mammalian -crystalline lens protein. Because of the estrogen responsive nature of HSP27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. Therefore, HSP27 may be useful in classifying various hormone sensitive tumors. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number
BNCB2244-100
Size / count
1x 100 µL
Dry ice
No
Hazardous
No
Discontinued
No
CAS Number
9007-83-4
Synonyms
Heat shock 27kDa protein; Estrogen-regulated 24kDa protein; Heat shock 25kDa protein 1; Heat shock 28kDa protein 1; Heat shock protein beta-1; HMN2B; Hsp25; Hsp28; HspB1; Stress-responsive protein 27 (SRP27)
UNSPSC
41116161
UNSPSC Description
Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name
HSPB1
Gene ID
3315
NCBI Gene ID
520973
UniProt
P04792
Swiss Prot
P04792
Host
Mouse
Species Reactivity
Human
Reactivity
Heat Shock 27kDa Protein 1
Immunogen
Recombinant human full-length protein
Target Antigen
Heat Shock 27kDa Protein 1
Clonality
Monoclonal
Clone
CPTC-HSPB1-2
Type
Primary
Source
Animal
Origin
Animal
Applications
Flow, intracellular (verified) |IHC, FFPE (verified) |WB (verified)
Positive Control
MCF-7, HeLa cells; ~50% of Breast Carcinomas are positive for hsp27 especially those that are also positive for ER and/or PgR.
Stability
2 years
Concentration
0.1 mg/mL
Buffer
PBS, 0.1% BSA, 0.05% azide
Molecular Weight
27 kDa
Shipping Conditions
RT (ND)
Storage Conditions
Stable at room temperature or 37°C (98°F) for 7 days.
Shelf Life
Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment
IgG1, kappa
Species
Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification
0.05% sodium azide (CAS 26628-22-8)
Conjugate
Biotin

Frequently Asked Questions

What is the catalog number for Heat Shock 27kDa Protein 1 (HPS27) (CPTC-HSPB1-2), Biotin conjugate, 0.1mg/mL?

The catalog number for Heat Shock 27kDa Protein 1 (HPS27) (CPTC-HSPB1-2), Biotin conjugate, 0.1mg/mL is BNCB2244-100.

What sizes are available for Heat Shock 27kDa Protein 1 (HPS27) (CPTC-HSPB1-2), Biotin conjugate, 0.1mg/mL?

Heat Shock 27kDa Protein 1 (HPS27) (CPTC-HSPB1-2), Biotin conjugate, 0.1mg/mL is available in the following size(s): 1x 100 µL.

Is Heat Shock 27kDa Protein 1 (HPS27) (CPTC-HSPB1-2), Biotin conjugate, 0.1mg/mL in stock?

Heat Shock 27kDa Protein 1 (HPS27) (CPTC-HSPB1-2), Biotin conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Heat Shock 27kDa Protein 1 (HPS27) (CPTC-HSPB1-2), Biotin conjugate, 0.1mg/mL?

Heat Shock 27kDa Protein 1 (HPS27) (CPTC-HSPB1-2), Biotin conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for Heat Shock 27kDa Protein 1 (HPS27) (CPTC-HSPB1-2), Biotin conjugate, 0.1mg/mL?

Estimated delivery time for Heat Shock 27kDa Protein 1 (HPS27) (CPTC-HSPB1-2), Biotin conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Heat Shock 27kDa Protein 1 (HPS27) (CPTC-HSPB1-2), Biotin conjugate, 0.1mg/mL?

The safety data sheet for Heat Shock 27kDa Protein 1 (HPS27) (CPTC-HSPB1-2), Biotin conjugate, 0.1mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is Heat Shock 27kDa Protein 1 (HPS27) (CPTC-HSPB1-2), Biotin conjugate, 0.1mg/mL used for?

HSP27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins produced by all organisms studied. HSP27 synthesis is induced by elevated temperature, as well as by estrogen in hormone responsive cells. Interestingly, human HSP27 also shares great

Is antigen retrieval required for IHC with this antibody?

Recommended antigen retrieval methods, where applicable, are listed on the datasheet under the IHC protocol section.

What temperature does this product ship at?

Shipping temperature is chosen based on the product’s stability needs, ranging from ambient to dry ice, and is indicated on the product page. Any related shipping fee is shown clearly in your quotation.

What is the molecular weight of the target protein?

Molecular weight information, where applicable, is listed on the datasheet under target details, useful for confirming the correct band on western blot.

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