Squamous Cell Carcinoma Antigen 1 (CPTC-SERPINB3-2), 0.2mg/mL

About this product

Metastasis of a primary tumor to a distant site is determined through signaling cascades that break down interactions between the cell and extracellular matrix proteins. Among the proteins mediating metastasis are serine proteases, such as neutrophil elastase. Serpins are family of serine protease inhibitors, contain a stretch of peptide that mimics a true substrate for a corresponding serine protease. Serine proteases bind to this substrate mimic in a 1:1 stoichiometric fashion and become catalytically inactive. Aberrant expression of serpin family members can contribute to a number of conditions, including emphysema (-1 antitrypsin deficiency), fatal bleeding (elastase to Thrombin specificity) and thrombosis (antithrombin deficiency), and are indicators of cancer stage phenotypes (circulating levels of squamous cell carcinoma antigen, known as SCCA1, increase in advancing stages of some cervical, lung, esophageal and head and neck cancers) . SCCA1 expression has been demonstrated to promote oncogenic transformation and epithelial-mesenchymal transition (EMT) in mammary epithelial cells, and its silencing in breast cancer cells has been shown to halt their proliferation. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNUB2250-500

Size / count

1x 500 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

HsT1196; Protein T4 A; Protein T4-A; SCC; SCCA-PD; SCCA1; SCCAPD; Serine (or cysteine) proteinase inhibitor clade B (ovalbumin) member 3; Serpin B3; SERPINB3; Squamous cell carcinoma antigen 1; T4 A; T4-A

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

SERPINB3

Gene ID

6317

NCBI Gene ID

227948

UniProt

P29508

Swiss Prot

P29508

Host

Mouse

Species Reactivity

Human

Reactivity

Squamous Cell Carcinoma Antigen 1

Immunogen

Recombinant human full-length SERPINB3 protein

Target Antigen

Squamous Cell Carcinoma Antigen 1

Clonality

Monoclonal

Clone

CPTC-SERPINB3-2

Type

Primary

Source

Animal

Origin

Animal

Applications

WB (published)

Positive Control

U-2197, HaCaT, Squamous cells. Expressed in some hepatocellular carcinoma.

Stability

2 years

Concentration

0.2 mg/mL

Buffer

PBS, 0.05% BSA, 0.05% azide

Molecular Weight

39 kDa

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. Wellcome Open Res (2018) 3: 51. (WB)

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG1, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

PBS, 0.05% BSA, 0.05% sodium azide (CAS 26628-22-8)

Conjugate

Purified, with BSA