Calcineurin B homologous protein 2 / HCC Antigen 520 (CPTC-CHP2-1), CF647 conjugate, 0.1mg/mL

About this product

Calcineurin B homologous protein 2, also known as hepatocellular carcinoma-associated antigen 520, is a 196 amino acid protein that contains 4 EF-hand domains and plays a potential role in transmembrane Na/H exchange. By binding to and activating NHE-1, CHP2 increases the pH and works to protect cells from serum deprivation-induced death. Though not typically detected in normal tissues, CHP2 is highly expressed in malignantly transformed cells and is therefore considered to be a tumor-associated antigen. Ectopic expression of CHP2 promotes proliferation of HEK293 cells and knockdown of CHP2 mRNA in HepG2 cells inhibits cell proliferation. Like calcineurin B, CHP2 can bind to and stimulate phosphatase activity of calcineurin A and activate the calcineurin/NFAT signaling pathway. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC472266-500

Size / count

1x 500 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

Calcineurin B homologous protein 2; Calcineurin-like EF hand protein 2; HCA520; hepatocellular carcinoma antigen gene 520

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

CHP2

Gene ID

63928

NCBI Gene ID

178589

UniProt

O43745

Swiss Prot

O43745

Host

Mouse

Species Reactivity

Human

Reactivity

Calcineurin B homologous protein 2|HCC Antigen 520

Immunogen

Recombinant human full-length CHP2 protein

Target Antigen

Calcineurin B homologous protein 2 | HCC Antigen 520

Clonality

Monoclonal

Clone

CPTC-CHP2-1

Type

Primary

Source

Animal

Origin

Animal

Applications

IF (verified) |WB (verified)

Field of Research

Ion channels

Positive Control

MCF-7, PC-3, HT29, K562 or Jurkat cell lysates. Human appendix or testis.

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

22 kDa

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG2c, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF647