HLA-DRB (MHC II) (DA2), CF594 conjugate, 0.1mg/mL

Catalog number BNC942281-500

Price for BNC942281-500

Size / count 1x 500 µL

About this product

This MAb reacts with a 28 kDa chain of HLA-DRB1 antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. The L243 antibody recognizes a different epitope than the LN3 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kDa alpha (heavy) chain and a 28 kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4 T cells. It is an excellent histiocytic marker in paraffin sections producing intense staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts. Loss of HLA-DR expression is related to tumor microenvironment and predicts adverse outcome in diffuse large B-cell lymphoma. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNC942281-500
Size / count: 1x 500 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: DRB1; HLA class II histocompatibility antigen; DR-1 beta chain; HLA-DR-beta 1; HLA-DRB1; human leucocyte antigen DRB1; Leucocyte antigen DR beta 1 chain; lymphocyte antigen DRB1; major histocompatibility complex; class II; DR beta 1; MHC class II HLA-DR beta 1 chain; MHC class II HLA-DR-beta cell surface glycoprotein
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: HLA-DRB1
Gene ID: 3123
NCBI Gene ID: 534322
UniProt: P01911
Swiss Prot: P01911
Host: Mouse
Species Reactivity: Human, Monkey
Reactivity: HLA-DRB
Immunogen: Activated human peripheral blood mononuclear cells
Target Antigen: HLA-DRB
Clonality: Monoclonal
Clone: DA2
Type: Primary
Source: Animal
Origin: Animal
Applications: IHC, FFPE (verified)
Positive Control: Ramos, Daudi or HuT78 cells. Tonsil or lymph node
Stability: 2 years
Concentration: 0.1 mg/mL
Buffer: PBS, 0.1% BSA, 0.05% azide
Molecular Weight: 28 kDa (beta chain)
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Notes: Protect from light
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG2b, kappa
Species: Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification: 0.05% sodium azide (CAS 26628-22-8)
Conjugate: CF594

Frequently Asked Questions

What is the catalog number for HLA-DRB (MHC II) (DA2), CF594 conjugate, 0.1mg/mL?

The catalog number for HLA-DRB (MHC II) (DA2), CF594 conjugate, 0.1mg/mL is BNC942281-500.

What sizes are available for HLA-DRB (MHC II) (DA2), CF594 conjugate, 0.1mg/mL?

HLA-DRB (MHC II) (DA2), CF594 conjugate, 0.1mg/mL is available in the following size(s): 1x 500 µL.

Is HLA-DRB (MHC II) (DA2), CF594 conjugate, 0.1mg/mL in stock?

HLA-DRB (MHC II) (DA2), CF594 conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures HLA-DRB (MHC II) (DA2), CF594 conjugate, 0.1mg/mL?

HLA-DRB (MHC II) (DA2), CF594 conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for HLA-DRB (MHC II) (DA2), CF594 conjugate, 0.1mg/mL?

Estimated delivery time for HLA-DRB (MHC II) (DA2), CF594 conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for HLA-DRB (MHC II) (DA2), CF594 conjugate, 0.1mg/mL?

The safety data sheet for HLA-DRB (MHC II) (DA2), CF594 conjugate, 0.1mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is HLA-DRB (MHC II) (DA2), CF594 conjugate, 0.1mg/mL used for?

Can this product be used for co-immunoprecipitation (Co-IP)?

Suitability for Co-IP depends on the specific antibody and is noted under validated applications on the datasheet. If it isn’t listed, contact our technical support team for guidance based on your target and sample type.

Is this kit compatible with automated liquid handling platforms?

Automation compatibility varies by kit and isn’t always listed on the datasheet. Contact our technical support team with details of your platform so we can confirm with the manufacturer.

What applications has this product been validated for?

Validated applications such as WB, ELISA, IHC, ICC, or FC are listed on the product datasheet. If you need application-specific validation data before ordering, contact our technical support team with the catalogue number.