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Product details

Collagen VII (LH7.2), CF640R conjugate, 0.1mg/mL

Catalog number BNC402315-100

Price for BNC402315-100

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Size / count 1x 100 µL

About this product

Recognizes an epitope located on collagenase digested Type VII collagen (150 kDa), i. e. the non-helical carboxyl terminal region of the dimer. Binding is localized at the inferior border of the lamina densa as demonstrated by immunoelectron microscopy. Type VII is also found in the retina, where its function is unknown. It interacts with Laminin 5 and Fibronectin. Collagen VII is a stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number
BNC402315-100
Size / count
1x 100 µL
Dry ice
No
Hazardous
No
Discontinued
No
CAS Number
9007-83-4
Synonyms
Alpha 1 type VII collagen; Collagen 7; Collagen alpha 1 (VII) chain; Collagen type VII alpha 1; Collagen VII alpha 1 polypeptide Col7a1; EBD1; EBDCT; EBR1; Long-chain (LC) collagen
UNSPSC
41116161
UNSPSC Description
Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name
COL7A1
Gene ID
1294
NCBI Gene ID
476218
UniProt
Q02388
Swiss Prot
Q02388
Host
Mouse
Species Reactivity
Cow, Goat, Guinea pig, Horse, Human, Sheep
Reactivity
Collagen VII
Immunogen
Recombinant full-length human Collagen VII protein
Target Antigen
Collagen VII
Clonality
Monoclonal
Clone
LH7.2
Type
Primary
Source
Animal
Origin
Animal
Applications
Flow, intracellular (verified) |IF (verified) |IHC, FFPE (verified)
Positive Control
HeLa, cells. Human lymphocytes. Human lymph node or tonsil.
Stability
2 years
Concentration
0.1 mg/mL
Buffer
PBS, 0.1% BSA, 0.05% azide
Molecular Weight
290 kDa
Shipping Conditions
RT (ND)
Storage Conditions
Stable at room temperature or 37°C (98°F) for 7 days.
Notes
Protect from light
Shelf Life
Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment
IgG2a, kappa
Species
Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification
0.05% sodium azide (CAS 26628-22-8)
Conjugate
CF640R

Frequently Asked Questions

What is the catalog number for Collagen VII (LH7.2), CF640R conjugate, 0.1mg/mL?

The catalog number for Collagen VII (LH7.2), CF640R conjugate, 0.1mg/mL is BNC402315-100.

What sizes are available for Collagen VII (LH7.2), CF640R conjugate, 0.1mg/mL?

Collagen VII (LH7.2), CF640R conjugate, 0.1mg/mL is available in the following size(s): 1x 100 µL.

Is Collagen VII (LH7.2), CF640R conjugate, 0.1mg/mL in stock?

Collagen VII (LH7.2), CF640R conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Collagen VII (LH7.2), CF640R conjugate, 0.1mg/mL?

Collagen VII (LH7.2), CF640R conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for Collagen VII (LH7.2), CF640R conjugate, 0.1mg/mL?

Estimated delivery time for Collagen VII (LH7.2), CF640R conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Collagen VII (LH7.2), CF640R conjugate, 0.1mg/mL?

The safety data sheet for Collagen VII (LH7.2), CF640R conjugate, 0.1mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is Collagen VII (LH7.2), CF640R conjugate, 0.1mg/mL used for?

Recognizes an epitope located on collagenase digested Type VII collagen (150 kDa), i. e. the non-helical carboxyl terminal region of the dimer. Binding is localized at the inferior border of the lamina densa as demonstrated by immunoelectron microscopy. Type VII is also found in the retina, where it

How long will it take to receive this product if it’s custom made?

Lead times depend on the product type: custom polyclonal antibodies typically take 12-14 weeks, monoclonal antibodies 15-17 weeks, made-to-order recombinant proteins 4-8 weeks, and made-to-order recombinant antibodies 6-8 weeks. These are estimates and may shift due to production delays.

What is the recommended dilution for this antibody?

A recommended dilution range for each validated application is provided on the datasheet, but optimal dilution should be titrated for your specific experimental system, sample type, and detection method.

What is the recommended storage temperature after reconstitution?

Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.

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