Collagen VII (LH7.2), Biotin conjugate, 0.1mg/mL

Catalog number BNCB2315-100

Price for BNCB2315-100

Size / count 1x 100 µL

About this product

Recognizes an epitope located on collagenase digested Type VII collagen (150 kDa), i. e. the non-helical carboxyl terminal region of the dimer. Binding is localized at the inferior border of the lamina densa as demonstrated by immunoelectron microscopy. Type VII is also found in the retina, where its function is unknown. It interacts with Laminin 5 and Fibronectin. Collagen VII is a stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNCB2315-100
Size / count: 1x 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: Alpha 1 type VII collagen; Collagen 7; Collagen alpha 1 (VII) chain; Collagen type VII alpha 1; Collagen VII alpha 1 polypeptide Col7a1; EBD1; EBDCT; EBR1; Long-chain (LC) collagen
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: COL7A1
Gene ID: 1294
NCBI Gene ID: 476218
UniProt: Q02388
Swiss Prot: Q02388
Host: Mouse
Species Reactivity: Cow, Goat, Guinea pig, Horse, Human, Sheep
Reactivity: Collagen VII
Immunogen: Recombinant full-length human Collagen VII protein
Target Antigen: Collagen VII
Clonality: Monoclonal
Clone: LH7.2
Type: Primary
Source: Animal
Origin: Animal
Applications: Flow, intracellular (verified) |IF (verified) |IHC, FFPE (verified)
Positive Control: HeLa, cells. Human lymphocytes. Human lymph node or tonsil.
Stability: 2 years
Concentration: 0.1 mg/mL
Buffer: PBS, 0.1% BSA, 0.05% azide
Molecular Weight: 290 kDa
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG2a, kappa
Species: Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification: 0.05% sodium azide (CAS 26628-22-8)
Conjugate: Biotin

Frequently Asked Questions

What is the catalog number for Collagen VII (LH7.2), Biotin conjugate, 0.1mg/mL?

The catalog number for Collagen VII (LH7.2), Biotin conjugate, 0.1mg/mL is BNCB2315-100.

What sizes are available for Collagen VII (LH7.2), Biotin conjugate, 0.1mg/mL?

Collagen VII (LH7.2), Biotin conjugate, 0.1mg/mL is available in the following size(s): 1x 100 µL.

Is Collagen VII (LH7.2), Biotin conjugate, 0.1mg/mL in stock?

Collagen VII (LH7.2), Biotin conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Collagen VII (LH7.2), Biotin conjugate, 0.1mg/mL?

Collagen VII (LH7.2), Biotin conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for Collagen VII (LH7.2), Biotin conjugate, 0.1mg/mL?

Estimated delivery time for Collagen VII (LH7.2), Biotin conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Collagen VII (LH7.2), Biotin conjugate, 0.1mg/mL?

The safety data sheet for Collagen VII (LH7.2), Biotin conjugate, 0.1mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is Collagen VII (LH7.2), Biotin conjugate, 0.1mg/mL used for?

What applications has this product been validated for?

Validated applications such as WB, ELISA, IHC, ICC, or FC are listed on the product datasheet. If you need application-specific validation data before ordering, contact our technical support team with the catalogue number.

How do I reconstitute this lyophilized product?

Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.