CD25 / IL2RA (Activated Lymphocyte Marker) (IL2RA/2394), CF405S conjugate, 0.1mg/mL

About this product

Recognizes a protein of 55 kDa, identified as CD25. It is expressed on activated T- and B-cells and activated monocytes/macrophages. With respect to lymphomas, CD25 is present on malignant cells of Hodgkin's disease, HTLV-1 adult T-cell leukemia, cutaneous T-cell lymphoma, and hair cell leukemia. Increased levels of soluble CD25 are observed in the leukemias/lymphomas and inflammatory/ autoimmune diseases. CD25 molecule alone appears to function as a low affinity receptor and associates with CD122 (IL-2R chain, p75) and CD132 (common chain) to form the high affinity IL-2 receptor complex. CD25 antibodies detect three epitope regions, A, B and C. This MAb recognizes the epitope B, which is located at residue 3-104 of CD25 and doe not block IL-2 binding to CD25. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC042394-500

Size / count

1x 500 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

IL2 Receptor alpha; IL2R alpha chain; IL2R; IL2R subunit alpha IL2RA; Interleukin-2 receptor subunit alpha; Ly43; p55; p55 chain; T Cell Growth Factor Receptor; TAC antigen; TCGFR

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

IL2RA

Gene ID

3559

NCBI Gene ID

231367

UniProt

P01589

Swiss Prot

P01589

Host

Mouse

Species Reactivity

Human

Reactivity

CD25|IL2RA

Immunogen

Recombinant human IL2RA protein fragment (around aa 42-183) (exact sequence is proprietary)

Target Antigen

CD25 | IL2RA

Clonality

Monoclonal

Clone

IL2RA/2394

Type

Primary

Source

Animal

Origin

Animal

Positive Control

PHA-stimulated human lymphocytes. Human lymph node or tonsil.

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

55 kDa

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG2b, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF405S