Perforin-1 (Pore Forming Protein) (Apoptosis Marker) (PRF1/2470), CF594 conjugate, 0.1mg/mL

Catalog number BNC942470-100

Price for BNC942470-100

Size / count 1x 100 µL

About this product

Perforin is a pore-forming protein that leads to osmotic lysis of the target cells and subsequently enables granzymes to enter the target cells and activate apoptosis. Perforin has structural and functional similarities to complement component 9 (C9) . Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. It is one of the main cytolytic proteins of cytolytic granules, and is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. The expression of perforin is reportedly upregulated in activated CD8 T-cells, natural killer cells and some CD4 T-cells. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNC942470-100
Size / count: 1x 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: Cytolysin; FLH2; HPLH2; Lymphocyte pore-forming protein; PRF1 (pore forming protein 1) ; Perforin-1; PFP; PGFL; PIGF; PIGF-2; PLGF
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: PRF1
Gene ID: 5551
NCBI Gene ID: 2200
UniProt: P14222
Swiss Prot: P14222
Host: Mouse
Species Reactivity: Human
Reactivity: Perforin-1
Immunogen: Recombinant human Perforin-1 protein fragment (around aa 413-552) (exact sequence is proprietary)
Target Antigen: Perforin-1
Clonality: Monoclonal
Clone: PRF1/2470
Type: Primary
Source: Animal
Origin: Animal
Applications: IHC, FFPE (verified)
Positive Control: HDLM-2 cells. Human spleen tissue (IHC).
Stability: 2 years
Concentration: 0.1 mg/mL
Buffer: PBS, 0.1% BSA, 0.05% azide
Molecular Weight: 75 kDa
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Notes: Protect from light
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG2c, kappa
Species: Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification: 0.05% sodium azide (CAS 26628-22-8)
Conjugate: CF594

Frequently Asked Questions

What is the catalog number for Perforin-1 (Pore Forming Protein) (Apoptosis Marker) (PRF1/2470), CF594 conjugate, 0.1mg/mL?

The catalog number for Perforin-1 (Pore Forming Protein) (Apoptosis Marker) (PRF1/2470), CF594 conjugate, 0.1mg/mL is BNC942470-100.

What sizes are available for Perforin-1 (Pore Forming Protein) (Apoptosis Marker) (PRF1/2470), CF594 conjugate, 0.1mg/mL?

Perforin-1 (Pore Forming Protein) (Apoptosis Marker) (PRF1/2470), CF594 conjugate, 0.1mg/mL is available in the following size(s): 1x 100 µL.

Is Perforin-1 (Pore Forming Protein) (Apoptosis Marker) (PRF1/2470), CF594 conjugate, 0.1mg/mL in stock?

Perforin-1 (Pore Forming Protein) (Apoptosis Marker) (PRF1/2470), CF594 conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Perforin-1 (Pore Forming Protein) (Apoptosis Marker) (PRF1/2470), CF594 conjugate, 0.1mg/mL?

Perforin-1 (Pore Forming Protein) (Apoptosis Marker) (PRF1/2470), CF594 conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for Perforin-1 (Pore Forming Protein) (Apoptosis Marker) (PRF1/2470), CF594 conjugate, 0.1mg/mL?

Estimated delivery time for Perforin-1 (Pore Forming Protein) (Apoptosis Marker) (PRF1/2470), CF594 conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Perforin-1 (Pore Forming Protein) (Apoptosis Marker) (PRF1/2470), CF594 conjugate, 0.1mg/mL?

The safety data sheet for Perforin-1 (Pore Forming Protein) (Apoptosis Marker) (PRF1/2470), CF594 conjugate, 0.1mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is Perforin-1 (Pore Forming Protein) (Apoptosis Marker) (PRF1/2470), CF594 conjugate, 0.1mg/mL used for?

What documentation should I request if this product is used for a regulated or intellectual-property-sensitive application?

If the product is subject to intellectual property protections, we’ll advise on any MTA or usage restrictions before shipping. Let us know your intended end use so we can confirm what’s required upfront.

Is this antibody conjugated?

Conjugation status, such as unconjugated, HRP, FITC, or biotin, is specified in the product name and confirmed on the datasheet.

What is the sensitivity of this ELISA kit?

Assay sensitivity, including the minimum detectable dose, is listed on the datasheet. Kits that are assembled on order typically ship within 2-4 weeks.