Perforin-1 (Pore Forming Protein) (Apoptosis Marker) (PRF1/2470), 1mg/mL

Catalog number BNUM2470-50

Price for BNUM2470-50

Size / count 1x 50 µL

About this product

Perforin is a pore-forming protein that leads to osmotic lysis of the target cells and subsequently enables granzymes to enter the target cells and activate apoptosis. Perforin has structural and functional similarities to complement component 9 (C9) . Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. It is one of the main cytolytic proteins of cytolytic granules, and is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. The expression of perforin is reportedly upregulated in activated CD8 T-cells, natural killer cells and some CD4 T-cells. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNUM2470-50
Size / count: 1x 50 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: Cytolysin; FLH2; HPLH2; Lymphocyte pore-forming protein; PRF1 (pore forming protein 1) ; Perforin-1; PFP; PGFL; PIGF; PIGF-2; PLGF
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: PRF1
Gene ID: 5551
NCBI Gene ID: 2200
UniProt: P14222
Swiss Prot: P14222
Host: Mouse
Species Reactivity: Human
Reactivity: Perforin-1
Immunogen: Recombinant human Perforin-1 protein fragment (around aa 413-552) (exact sequence is proprietary)
Target Antigen: Perforin-1
Clonality: Monoclonal
Clone: PRF1/2470
Type: Primary
Source: Animal
Origin: Animal
Applications: IHC, FFPE (verified)
Positive Control: HDLM-2 cells. Human spleen tissue (IHC).
Stability: 2 years
Concentration: 1 mg/mL
Buffer: PBS, no BSA, no azide
Molecular Weight: 75 kDa
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG2c, kappa
Species: Mus musculus (mouse)
Classification: PBS, no BSA, no sodium azide (CAS 26628-22-8)
Conjugate: Purified, BSA-free

Frequently Asked Questions

What is the catalog number for Perforin-1 (Pore Forming Protein) (Apoptosis Marker) (PRF1/2470), 1mg/mL?

The catalog number for Perforin-1 (Pore Forming Protein) (Apoptosis Marker) (PRF1/2470), 1mg/mL is BNUM2470-50.

What sizes are available for Perforin-1 (Pore Forming Protein) (Apoptosis Marker) (PRF1/2470), 1mg/mL?

Perforin-1 (Pore Forming Protein) (Apoptosis Marker) (PRF1/2470), 1mg/mL is available in the following size(s): 1x 50 µL.

Is Perforin-1 (Pore Forming Protein) (Apoptosis Marker) (PRF1/2470), 1mg/mL in stock?

Perforin-1 (Pore Forming Protein) (Apoptosis Marker) (PRF1/2470), 1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Perforin-1 (Pore Forming Protein) (Apoptosis Marker) (PRF1/2470), 1mg/mL?

Perforin-1 (Pore Forming Protein) (Apoptosis Marker) (PRF1/2470), 1mg/mL is manufactured by Biotium.

What is the estimated delivery time for Perforin-1 (Pore Forming Protein) (Apoptosis Marker) (PRF1/2470), 1mg/mL?

Estimated delivery time for Perforin-1 (Pore Forming Protein) (Apoptosis Marker) (PRF1/2470), 1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Perforin-1 (Pore Forming Protein) (Apoptosis Marker) (PRF1/2470), 1mg/mL?

The safety data sheet for Perforin-1 (Pore Forming Protein) (Apoptosis Marker) (PRF1/2470), 1mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is Perforin-1 (Pore Forming Protein) (Apoptosis Marker) (PRF1/2470), 1mg/mL used for?

What is the purity of this recombinant protein?

Purity, typically assessed by SDS-PAGE, is listed on the datasheet under quality control data.

What do I do if this product arrives with reduced ice or a damaged cold-chain package?

Report this to us immediately with photos and your order reference. Our specialists will assess whether the product is still usable given the cold-chain interruption, and will arrange a replacement or credit if it isn’t.

What host species was used to raise this antibody?

The host species used in production is listed under product specifications on the datasheet, and is relevant when selecting a compatible secondary antibody for your assay.