AKT1 (Prognostic Marker for Neuroendocrine Tumors) (AKT1/2491), CF594 conjugate, 0.1mg/mL

Catalog number BNC942491-100

Price for BNC942491-100

Size / count 1x 100 µL

About this product

AKT1 is a 62 kDa protein and member of the serine/threonine kinase AKT family. AKT1 is also known as Protein Kinase B (PKB) or Rac Protein Kinase Alpha. The Akt family also includes AKT2. Activation of AKT1 by insulin or insulin-growth factor-1 (IGF-1) results in phosphorylation of both Thr 308 and Ser 473. AKT proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase (s), and the activation of AKT1 and AKT2 is inhibited by the PI kinase inhibitor wortmannin. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNC942491-100
Size / count: 1x 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: AKT1; PKB; Protein Kinase B Alpha; RAC-alpha serine/threonine-protein kinase
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: AKT1
Gene ID: 207
NCBI Gene ID: 525622
UniProt: P31749
Swiss Prot: P31749
Host: Mouse
Species Reactivity: Human
Reactivity: AKT1
Immunogen: Recombinant fragment of human AKT1 protein (around aa 85-189) (exact sequence is proprietary)
Target Antigen: AKT1
Clonality: Monoclonal
Clone: AKT1/2491
Type: Primary
Source: Animal
Origin: Animal
Applications: IHC, FFPE (verified) |WB (verified)
Positive Control: PDGF-treated NIH/3T3 cells. HeLa cell lysates. Human pancreas or cervical carcinoma.
Stability: 2 years
Concentration: 0.1 mg/mL
Buffer: PBS, 0.1% BSA, 0.05% azide
Molecular Weight: 62 kDa
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Notes: Protect from light
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG2b, kappa
Species: Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification: 0.05% sodium azide (CAS 26628-22-8)
Conjugate: CF594

Frequently Asked Questions

What is the catalog number for AKT1 (Prognostic Marker for Neuroendocrine Tumors) (AKT1/2491), CF594 conjugate, 0.1mg/mL?

The catalog number for AKT1 (Prognostic Marker for Neuroendocrine Tumors) (AKT1/2491), CF594 conjugate, 0.1mg/mL is BNC942491-100.

What sizes are available for AKT1 (Prognostic Marker for Neuroendocrine Tumors) (AKT1/2491), CF594 conjugate, 0.1mg/mL?

AKT1 (Prognostic Marker for Neuroendocrine Tumors) (AKT1/2491), CF594 conjugate, 0.1mg/mL is available in the following size(s): 1x 100 µL.

Is AKT1 (Prognostic Marker for Neuroendocrine Tumors) (AKT1/2491), CF594 conjugate, 0.1mg/mL in stock?

AKT1 (Prognostic Marker for Neuroendocrine Tumors) (AKT1/2491), CF594 conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures AKT1 (Prognostic Marker for Neuroendocrine Tumors) (AKT1/2491), CF594 conjugate, 0.1mg/mL?

AKT1 (Prognostic Marker for Neuroendocrine Tumors) (AKT1/2491), CF594 conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for AKT1 (Prognostic Marker for Neuroendocrine Tumors) (AKT1/2491), CF594 conjugate, 0.1mg/mL?

Estimated delivery time for AKT1 (Prognostic Marker for Neuroendocrine Tumors) (AKT1/2491), CF594 conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for AKT1 (Prognostic Marker for Neuroendocrine Tumors) (AKT1/2491), CF594 conjugate, 0.1mg/mL?

The safety data sheet for AKT1 (Prognostic Marker for Neuroendocrine Tumors) (AKT1/2491), CF594 conjugate, 0.1mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is AKT1 (Prognostic Marker for Neuroendocrine Tumors) (AKT1/2491), CF594 conjugate, 0.1mg/mL used for?

What applications has this product been validated for?

Validated applications such as WB, ELISA, IHC, ICC, or FC are listed on the product datasheet. If you need application-specific validation data before ordering, contact our technical support team with the catalogue number.

How do I reconstitute this lyophilized product?

Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.