CD73 (Immuno-Oncology Target) (NT5E/2505), CF488A conjugate, 0.1mg/mL

Catalog number BNC882505-500

Price for BNC882505-500

Size / count 1x 500 µL

About this product

CD73 (also designated ecto-5'-nucleotidase, E5NT, NT, NT5, NTE, eN and eNT) is a glycosyl-phosphatidylinositol (GPI) -anchored adhesion protein that catalyzes the dephosphorylation of extracellular purine and pyrimidine nucleotides to their corresponding bioactive nucleosides. CD73 is a dimer of two identical subunits that depends on GPI to link with the external face of the plasma membrane. Similar to other GPI-anchored proteins, CD73 mediates co-stimulatory signals in T cell activation. CD73 has few structural variants, yet elicits diverse biological function through differential regulation in endothelial cells (EC), subpopulations of B and T cells, germinal center follicular dendritic cells and on thymic medullary reticular fibroblasts. For example, IgG mediated neutralization of CD73 interferes with lymphocyte adhesion to EC, and blocks aggregation of germinal center B cells and follicular dendritic cells. Furthermore, IgG-mediated targeting of lymphocyte CD73, but not of endothelial cell CD73, causes shedding of CD73 and tyrosine phosphorylation of proteins. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNC882505-500
Size / count: 1x 500 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: 5' NT; 5' nucleotidase (CD73) ; 5' nucleotidase precursor; 5' nucleotidase; ecto; 5' nucleotidase; ecto (CD73) ; 5'-NT; 5'-nucleotidase; 5NTD_HUMAN; CD73; CD73 antigen; E5NT; Ecto 5' nucleotidase; Ecto-5'-nucleotidase; eN; eNT; NT; NT5; NT5E; NTE; Purine 5 Prime Nucleotidase
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: NT5E
Gene ID: 4907
NCBI Gene ID: 153952
UniProt: P21589
Swiss Prot: P21589
Host: Mouse
Species Reactivity: Human
Reactivity: CD73
Immunogen: Recombinant full length human NT5E protein
Target Antigen: CD73
Clonality: Monoclonal
Clone: NT5E/2505
Type: Primary
Source: Animal
Origin: Animal
Applications: Flow (verified) |IF (verified) |IHC, FFPE (verified) |WB (verified)
Positive Control: Human tonsil, prostate or lung carcinoma.
Stability: 2 years
Concentration: 0.1 mg/mL
Buffer: PBS, 0.1% BSA, 0.05% azide
Molecular Weight: 71 kDa
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Notes: Protect from light
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG1, kappa
Species: Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification: 0.05% sodium azide (CAS 26628-22-8)
Conjugate: CF488A

Frequently Asked Questions

What is the catalog number for CD73 (Immuno-Oncology Target) (NT5E/2505), CF488A conjugate, 0.1mg/mL?

The catalog number for CD73 (Immuno-Oncology Target) (NT5E/2505), CF488A conjugate, 0.1mg/mL is BNC882505-500.

What sizes are available for CD73 (Immuno-Oncology Target) (NT5E/2505), CF488A conjugate, 0.1mg/mL?

CD73 (Immuno-Oncology Target) (NT5E/2505), CF488A conjugate, 0.1mg/mL is available in the following size(s): 1x 500 µL.

Is CD73 (Immuno-Oncology Target) (NT5E/2505), CF488A conjugate, 0.1mg/mL in stock?

CD73 (Immuno-Oncology Target) (NT5E/2505), CF488A conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures CD73 (Immuno-Oncology Target) (NT5E/2505), CF488A conjugate, 0.1mg/mL?

CD73 (Immuno-Oncology Target) (NT5E/2505), CF488A conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for CD73 (Immuno-Oncology Target) (NT5E/2505), CF488A conjugate, 0.1mg/mL?

Estimated delivery time for CD73 (Immuno-Oncology Target) (NT5E/2505), CF488A conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for CD73 (Immuno-Oncology Target) (NT5E/2505), CF488A conjugate, 0.1mg/mL?

The safety data sheet for CD73 (Immuno-Oncology Target) (NT5E/2505), CF488A conjugate, 0.1mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is CD73 (Immuno-Oncology Target) (NT5E/2505), CF488A conjugate, 0.1mg/mL used for?

What buffer is this product supplied in?

The formulation or storage buffer is listed on the product datasheet. If you require a carrier-free or custom formulation, contact us before ordering, as this may involve a made-to-order production timeline.

What dilution range is recommended for immunohistochemistry (IHC) with this antibody?

A starting IHC dilution range is listed on the datasheet, but should be optimized for your specific tissue type and staining protocol.

Can I request a Safety Data Sheet (SDS) for this product?

Yes, safety data sheets are available for hazardous or chemically classified products and can be provided on request or downloaded from the product page.