CD73 (Immuno-Oncology Target) (NT5E/2505), 0.2mg/mL

Catalog number BNUB2505-100

Price for BNUB2505-100

Size / count 1x 100 µL

About this product

CD73 (also designated ecto-5'-nucleotidase, E5NT, NT, NT5, NTE, eN and eNT) is a glycosyl-phosphatidylinositol (GPI) -anchored adhesion protein that catalyzes the dephosphorylation of extracellular purine and pyrimidine nucleotides to their corresponding bioactive nucleosides. CD73 is a dimer of two identical subunits that depends on GPI to link with the external face of the plasma membrane. Similar to other GPI-anchored proteins, CD73 mediates co-stimulatory signals in T cell activation. CD73 has few structural variants, yet elicits diverse biological function through differential regulation in endothelial cells (EC), subpopulations of B and T cells, germinal center follicular dendritic cells and on thymic medullary reticular fibroblasts. For example, IgG mediated neutralization of CD73 interferes with lymphocyte adhesion to EC, and blocks aggregation of germinal center B cells and follicular dendritic cells. Furthermore, IgG-mediated targeting of lymphocyte CD73, but not of endothelial cell CD73, causes shedding of CD73 and tyrosine phosphorylation of proteins. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNUB2505-100
Size / count: 1x 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: 5' NT; 5' nucleotidase (CD73) ; 5' nucleotidase precursor; 5' nucleotidase; ecto; 5' nucleotidase; ecto (CD73) ; 5'-NT; 5'-nucleotidase; 5NTD_HUMAN; CD73; CD73 antigen; E5NT; Ecto 5' nucleotidase; Ecto-5'-nucleotidase; eN; eNT; NT; NT5; NT5E; NTE; Purine 5 Prime Nucleotidase
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: NT5E
Gene ID: 4907
NCBI Gene ID: 153952
UniProt: P21589
Swiss Prot: P21589
Host: Mouse
Species Reactivity: Human
Reactivity: CD73
Immunogen: Recombinant full length human NT5E protein
Target Antigen: CD73
Clonality: Monoclonal
Clone: NT5E/2505
Type: Primary
Source: Animal
Origin: Animal
Applications: Flow (verified) |IF (verified) |IHC, FFPE (verified) |WB (verified)
Positive Control: Human tonsil, prostate or lung carcinoma.
Stability: 2 years
Concentration: 0.2 mg/mL
Buffer: PBS, 0.05% BSA, 0.05% azide
Molecular Weight: 71 kDa
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG1, kappa
Species: Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification: PBS, 0.05% BSA, 0.05% sodium azide (CAS 26628-22-8)
Conjugate: Purified, with BSA

Frequently Asked Questions

What is the catalog number for CD73 (Immuno-Oncology Target) (NT5E/2505), 0.2mg/mL?

The catalog number for CD73 (Immuno-Oncology Target) (NT5E/2505), 0.2mg/mL is BNUB2505-100.

What sizes are available for CD73 (Immuno-Oncology Target) (NT5E/2505), 0.2mg/mL?

CD73 (Immuno-Oncology Target) (NT5E/2505), 0.2mg/mL is available in the following size(s): 1x 100 µL.

Is CD73 (Immuno-Oncology Target) (NT5E/2505), 0.2mg/mL in stock?

CD73 (Immuno-Oncology Target) (NT5E/2505), 0.2mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures CD73 (Immuno-Oncology Target) (NT5E/2505), 0.2mg/mL?

CD73 (Immuno-Oncology Target) (NT5E/2505), 0.2mg/mL is manufactured by Biotium.

What is the estimated delivery time for CD73 (Immuno-Oncology Target) (NT5E/2505), 0.2mg/mL?

Estimated delivery time for CD73 (Immuno-Oncology Target) (NT5E/2505), 0.2mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for CD73 (Immuno-Oncology Target) (NT5E/2505), 0.2mg/mL?

The safety data sheet for CD73 (Immuno-Oncology Target) (NT5E/2505), 0.2mg/mL is available here: https://cdn.gentaur.com/products/37/68dd0297061270361e10029d/msds/bnub2505-100.pdf.

What is CD73 (Immuno-Oncology Target) (NT5E/2505), 0.2mg/mL used for?

What applications has this product been validated for?

Validated applications such as WB, ELISA, IHC, ICC, or FC are listed on the product datasheet. If you need application-specific validation data before ordering, contact our technical support team with the catalogue number.

How do I reconstitute this lyophilized product?

Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.