CD73 (Immuno-Oncology Target) (NT5E/2545), 1mg/mL

About this product

CD73 (also designated ecto-5'-nucleotidase, E5NT, NT, NT5, NTE, eN and eNT) is a glycosyl-phosphatidylinositol (GPI) -anchored adhesion protein that catalyzes the dephosphorylation of extracellular purine and pyrimidine nucleotides to their corresponding bioactive nucleosides. CD73 is a dimer of two identical subunits that depends on GPI to link with the external face of the plasma membrane. Similar to other GPI-anchored proteins, CD73 mediates co-stimulatory signals in T cell activation. CD73 has few structural variants, yet elicits diverse biological function through differential regulation in endothelial cells (EC), subpopulations of B and T cells, germinal center follicular dendritic cells and on thymic medullary reticular fibroblasts. For example, IgG mediated neutralization of CD73 interferes with lymphocyte adhesion to EC, and blocks aggregation of germinal center B cells and follicular dendritic cells. Furthermore, IgG-mediated targeting of lymphocyte CD73, but not of endothelial cell CD73, causes shedding of CD73 and tyrosine phosphorylation of proteins. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNUM2545-50

Size / count

1x 50 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

5' NT; 5' nucleotidase (CD73) ; 5' nucleotidase precursor; 5' nucleotidase; ecto; 5' nucleotidase; ecto (CD73) ; 5'-NT; 5'-nucleotidase; 5NTD_HUMAN; CD73; CD73 antigen; E5NT; Ecto 5' nucleotidase; Ecto-5'-nucleotidase; eN; eNT; NT; NT5; NT5E; NTE; Purine 5 Prime Nucleotidase

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

NT5E

Gene ID

4907

NCBI Gene ID

153952

UniProt

P21589

Swiss Prot

P21589

Host

Mouse

Species Reactivity

Human

Reactivity

CD73

Immunogen

Recombinant full length human NT5E protein

Target Antigen

CD73

Clonality

Monoclonal

Clone

NT5E/2545

Type

Primary

Source

Animal

Origin

Animal

Applications

Flow (verified) |IF (verified) |IHC, FFPE (verified) |WB (verified)

Positive Control

Tonsil, Prostate or Lung Carcinoma.

Stability

2 years

Concentration

1 mg/mL

Buffer

PBS, no BSA, no azide

Molecular Weight

71 kDa

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG1, kappa

Species

Mus musculus (mouse)

Classification

PBS, no BSA, no sodium azide (CAS 26628-22-8)

Conjugate

Purified, BSA-free