ATG5 (Autophagy Marker) (ATG5/2553), CF647 conjugate, 0.1mg/mL

About this product

Autophagy protein 5 (ATG5), in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. ATG5 is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. The ATG5 protein is essential for autophagy; a process that is usually beneficial for cells to self-degrade their own components when they are no longer useful. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC472553-500

Size / count

1x 500 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

Autophagy protein 5; ATG5; Autophagy related 5; APG5-like; Apoptosis-specific protein

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

ATG5

Gene ID

9474

NCBI Gene ID

486063

UniProt

Q9H1Y0

Swiss Prot

Q9H1Y0

Host

Mouse

Species Reactivity

Human

Reactivity

ATG5|Autophagy protein 5

Immunogen

Recombinant fragment of human ATG5 protein (around aa 1-119) (exact sequence is proprietary)

Target Antigen

ATG5 | Autophagy protein 5

Clonality

Monoclonal

Clone

ATG5/2553

Type

Primary

Source

Animal

Origin

Animal

Applications

IHC, FFPE (verified) |WB (verified)

Positive Control

K562, U-87, A431, THP-1, PANC-1, Raji or HeLa cells. Ovary, Endometrium, Colon or Duodenum.

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

32 kDa

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG1, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF647