ATG5 (Autophagy Marker) (ATG5/2553), Biotin conjugate, 0.1mg/mL

Catalog number BNCB2553-100

Price for BNCB2553-100

Size / count 1x 100 µL

About this product

Autophagy protein 5 (ATG5), in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. ATG5 is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. The ATG5 protein is essential for autophagy; a process that is usually beneficial for cells to self-degrade their own components when they are no longer useful. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNCB2553-100
Size / count: 1x 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: Autophagy protein 5; ATG5; Autophagy related 5; APG5-like; Apoptosis-specific protein
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: ATG5
Gene ID: 9474
NCBI Gene ID: 486063
UniProt: Q9H1Y0
Swiss Prot: Q9H1Y0
Host: Mouse
Species Reactivity: Human
Reactivity: ATG5|Autophagy protein 5
Immunogen: Recombinant fragment of human ATG5 protein (around aa 1-119) (exact sequence is proprietary)
Target Antigen: ATG5 | Autophagy protein 5
Clonality: Monoclonal
Clone: ATG5/2553
Type: Primary
Source: Animal
Origin: Animal
Applications: IHC, FFPE (verified) |WB (verified)
Positive Control: K562, U-87, A431, THP-1, PANC-1, Raji or HeLa cells. Ovary, Endometrium, Colon or Duodenum.
Stability: 2 years
Concentration: 0.1 mg/mL
Buffer: PBS, 0.1% BSA, 0.05% azide
Molecular Weight: 32 kDa
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG1, kappa
Species: Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification: 0.05% sodium azide (CAS 26628-22-8)
Conjugate: Biotin

Frequently Asked Questions

What is the catalog number for ATG5 (Autophagy Marker) (ATG5/2553), Biotin conjugate, 0.1mg/mL?

The catalog number for ATG5 (Autophagy Marker) (ATG5/2553), Biotin conjugate, 0.1mg/mL is BNCB2553-100.

What sizes are available for ATG5 (Autophagy Marker) (ATG5/2553), Biotin conjugate, 0.1mg/mL?

ATG5 (Autophagy Marker) (ATG5/2553), Biotin conjugate, 0.1mg/mL is available in the following size(s): 1x 100 µL.

Is ATG5 (Autophagy Marker) (ATG5/2553), Biotin conjugate, 0.1mg/mL in stock?

ATG5 (Autophagy Marker) (ATG5/2553), Biotin conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures ATG5 (Autophagy Marker) (ATG5/2553), Biotin conjugate, 0.1mg/mL?

ATG5 (Autophagy Marker) (ATG5/2553), Biotin conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for ATG5 (Autophagy Marker) (ATG5/2553), Biotin conjugate, 0.1mg/mL?

Estimated delivery time for ATG5 (Autophagy Marker) (ATG5/2553), Biotin conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for ATG5 (Autophagy Marker) (ATG5/2553), Biotin conjugate, 0.1mg/mL?

The safety data sheet for ATG5 (Autophagy Marker) (ATG5/2553), Biotin conjugate, 0.1mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is ATG5 (Autophagy Marker) (ATG5/2553), Biotin conjugate, 0.1mg/mL used for?

What is the recommended storage temperature after reconstitution?

Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.

Can this product be used in multiplex assays?

Multiplex compatibility depends on the specific product and detection panel. Contact our technical support team with details of your multiplex system for guidance before ordering.

What happens if this product doesn’t perform as expected in my experiment?

Send us the catalogue number, product name, lot number, and a summary of your results. Our technical support team will investigate and, where needed, involve the manufacturer. This is covered under our guarantee provided the product was used before its expiry date and stored correctly.