CD73 (Immuno-Oncology Target) (NT5E/2646), CF640R conjugate, 0.1mg/mL

Catalog number BNC402646-500

Price for BNC402646-500

Size / count 1x 500 µL

About this product

CD73 is a membrane-bound extracellular enzyme overexpressed in several cancer types. Its expression has been linked to poor prognosis in melanoma, colorectal, gastric, triple negative breast cancer, and to a pro-metastatic phenotype in prostate cancer. Together with CD39, it plays a major role in promoting immunosuppression through the pathway degrading adenosine triphosphate (ATP) into adenosine. Within the tumor microenvironment, ATP promotes immune cell-mediated killing of cancer cells. In contrast, adenosine accumulation causes immune suppression, dysregulation of immune cell infiltrates and stimulates angiogenesis resulting in tumor spreading. CD73 is active on the last step of the degradation pathway, where it is the enzyme that actually degrades AMP into adenosine. CD73-blockade promotes anti-tumor immunity by reducing adenosine accumulation. Accordingly, anti-CD73 mAbs stimulate anti-tumor immunity and reduce tumor metastasis in Mouse tumor models, and could enhance the efficacy of treatment with anti-PD1 or anti-CTLA4 antibodies. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNC402646-500
Size / count: 1x 500 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: 5' NT; 5' nucleotidase (CD73) ; 5' nucleotidase precursor; 5' nucleotidase; ecto; 5' nucleotidase; ecto (CD73) ; E5NT; Ecto 5' nucleotidase; Ecto-5'-nucleotidase; eN; eNT; NT; NT5; NT5E; NTE; Purine 5 Prime Nucleotidase
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: NT5E
Gene ID: 4907
NCBI Gene ID: 153952
UniProt: P21589
Swiss Prot: P21589
Host: Mouse
Species Reactivity: Human
Reactivity: CD73
Immunogen: Recombinant full length human NT5E protein
Target Antigen: CD73
Clonality: Monoclonal
Clone: NT5E/2646
Type: Primary
Source: Animal
Origin: Animal
Applications: Flow (verified) |IF (verified) |IHC, FFPE (verified) |WB (verified)
Positive Control: A431, U87MG, PC-3 cells. Tonsil, Prostate or Lung Carcinoma.
Stability: 2 years
Concentration: 0.1 mg/mL
Buffer: PBS, 0.1% BSA, 0.05% azide
Molecular Weight: 71 kDa
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Notes: Protect from light
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG1, kappa
Species: Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification: 0.05% sodium azide (CAS 26628-22-8)
Conjugate: CF640R

Frequently Asked Questions

What is the catalog number for CD73 (Immuno-Oncology Target) (NT5E/2646), CF640R conjugate, 0.1mg/mL?

The catalog number for CD73 (Immuno-Oncology Target) (NT5E/2646), CF640R conjugate, 0.1mg/mL is BNC402646-500.

What sizes are available for CD73 (Immuno-Oncology Target) (NT5E/2646), CF640R conjugate, 0.1mg/mL?

CD73 (Immuno-Oncology Target) (NT5E/2646), CF640R conjugate, 0.1mg/mL is available in the following size(s): 1x 500 µL.

Is CD73 (Immuno-Oncology Target) (NT5E/2646), CF640R conjugate, 0.1mg/mL in stock?

CD73 (Immuno-Oncology Target) (NT5E/2646), CF640R conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures CD73 (Immuno-Oncology Target) (NT5E/2646), CF640R conjugate, 0.1mg/mL?

CD73 (Immuno-Oncology Target) (NT5E/2646), CF640R conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for CD73 (Immuno-Oncology Target) (NT5E/2646), CF640R conjugate, 0.1mg/mL?

Estimated delivery time for CD73 (Immuno-Oncology Target) (NT5E/2646), CF640R conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for CD73 (Immuno-Oncology Target) (NT5E/2646), CF640R conjugate, 0.1mg/mL?

The safety data sheet for CD73 (Immuno-Oncology Target) (NT5E/2646), CF640R conjugate, 0.1mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is CD73 (Immuno-Oncology Target) (NT5E/2646), CF640R conjugate, 0.1mg/mL used for?

Is antigen retrieval required for IHC with this antibody?

Recommended antigen retrieval methods, where applicable, are listed on the datasheet under the IHC protocol section.

What temperature does this product ship at?

Shipping temperature is chosen based on the product’s stability needs, ranging from ambient to dry ice, and is indicated on the product page. Any related shipping fee is shown clearly in your quotation.

What is the molecular weight of the target protein?

Molecular weight information, where applicable, is listed on the datasheet under target details, useful for confirming the correct band on western blot.