CD73 (Immuno-Oncology Target) (NT5E/2646), CF488A conjugate, 0.1mg/mL

Catalog number BNC882646-500

Price for BNC882646-500

Size / count 1x 500 µL

About this product

CD73 is a membrane-bound extracellular enzyme overexpressed in several cancer types. Its expression has been linked to poor prognosis in melanoma, colorectal, gastric, triple negative breast cancer, and to a pro-metastatic phenotype in prostate cancer. Together with CD39, it plays a major role in promoting immunosuppression through the pathway degrading adenosine triphosphate (ATP) into adenosine. Within the tumor microenvironment, ATP promotes immune cell-mediated killing of cancer cells. In contrast, adenosine accumulation causes immune suppression, dysregulation of immune cell infiltrates and stimulates angiogenesis resulting in tumor spreading. CD73 is active on the last step of the degradation pathway, where it is the enzyme that actually degrades AMP into adenosine. CD73-blockade promotes anti-tumor immunity by reducing adenosine accumulation. Accordingly, anti-CD73 mAbs stimulate anti-tumor immunity and reduce tumor metastasis in Mouse tumor models, and could enhance the efficacy of treatment with anti-PD1 or anti-CTLA4 antibodies. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNC882646-500
Size / count: 1x 500 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: 5' NT; 5' nucleotidase (CD73) ; 5' nucleotidase precursor; 5' nucleotidase; ecto; 5' nucleotidase; ecto (CD73) ; E5NT; Ecto 5' nucleotidase; Ecto-5'-nucleotidase; eN; eNT; NT; NT5; NT5E; NTE; Purine 5 Prime Nucleotidase
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: NT5E
Gene ID: 4907
NCBI Gene ID: 153952
UniProt: P21589
Swiss Prot: P21589
Host: Mouse
Species Reactivity: Human
Reactivity: CD73
Immunogen: Recombinant full length human NT5E protein
Target Antigen: CD73
Clonality: Monoclonal
Clone: NT5E/2646
Type: Primary
Source: Animal
Origin: Animal
Applications: Flow (verified) |IF (verified) |IHC, FFPE (verified) |WB (verified)
Positive Control: A431, U87MG, PC-3 cells. Tonsil, Prostate or Lung Carcinoma.
Stability: 2 years
Concentration: 0.1 mg/mL
Buffer: PBS, 0.1% BSA, 0.05% azide
Molecular Weight: 71 kDa
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Notes: Protect from light
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG1, kappa
Species: Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification: 0.05% sodium azide (CAS 26628-22-8)
Conjugate: CF488A

Frequently Asked Questions

What is the catalog number for CD73 (Immuno-Oncology Target) (NT5E/2646), CF488A conjugate, 0.1mg/mL?

The catalog number for CD73 (Immuno-Oncology Target) (NT5E/2646), CF488A conjugate, 0.1mg/mL is BNC882646-500.

What sizes are available for CD73 (Immuno-Oncology Target) (NT5E/2646), CF488A conjugate, 0.1mg/mL?

CD73 (Immuno-Oncology Target) (NT5E/2646), CF488A conjugate, 0.1mg/mL is available in the following size(s): 1x 500 µL.

Is CD73 (Immuno-Oncology Target) (NT5E/2646), CF488A conjugate, 0.1mg/mL in stock?

CD73 (Immuno-Oncology Target) (NT5E/2646), CF488A conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures CD73 (Immuno-Oncology Target) (NT5E/2646), CF488A conjugate, 0.1mg/mL?

CD73 (Immuno-Oncology Target) (NT5E/2646), CF488A conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for CD73 (Immuno-Oncology Target) (NT5E/2646), CF488A conjugate, 0.1mg/mL?

Estimated delivery time for CD73 (Immuno-Oncology Target) (NT5E/2646), CF488A conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for CD73 (Immuno-Oncology Target) (NT5E/2646), CF488A conjugate, 0.1mg/mL?

The safety data sheet for CD73 (Immuno-Oncology Target) (NT5E/2646), CF488A conjugate, 0.1mg/mL is available here: https://cdn.gentaur.com/products/37/68dd029d061270361e102626/msds/bnc882646-500.pdf.

What is CD73 (Immuno-Oncology Target) (NT5E/2646), CF488A conjugate, 0.1mg/mL used for?

How do I reconstitute this lyophilized product?

Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.

What should I check when this product first arrives?

Inspect the package immediately for transportation damage, ice loss, leakage, or breakage. Report any issues right away with photos and your order reference; our specialists will determine whether the product is still usable or needs replacing.