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Product details

Vimentin (Mesenchymal Cell Marker) (V9), CF514 conjugate, 0.1mg/mL

Catalog number BNC142775-100

Price for BNC142775-100

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Size / count 1x 100 µL

About this product

This MAb reacts with a 58 kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Anti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e. g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number
BNC142775-100
Size / count
1x 100 µL
Dry ice
No
Hazardous
No
Discontinued
No
CAS Number
9007-83-4
Synonyms
VIM
UNSPSC
41116161
UNSPSC Description
Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name
VIM
Gene ID
7431
NCBI Gene ID
455493
UniProt
P08670
Swiss Prot
P08670
Host
Mouse
Species Reactivity
Human
Reactivity
Vimentin
Immunogen
Porcine Lens
Target Antigen
Vimentin
Clonality
Monoclonal
Clone
V9
Type
Primary
Source
Animal
Origin
Animal
Applications
IHC, FFPE (verified) |WB (verified)
Positive Control
U-87, Raji, Jurkat or HeLa cells. Sarcomas or Melanomas.
Stability
2 years
Concentration
0.1 mg/mL
Buffer
PBS, 0.1% BSA, 0.05% azide
Molecular Weight
57-60 kDa
Shipping Conditions
RT (ND)
Storage Conditions
Stable at room temperature or 37°C (98°F) for 7 days.
Notes
Protect from light
Shelf Life
Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment
IgG1, kappa
Species
Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification
0.05% sodium azide (CAS 26628-22-8)
Conjugate
CF514

Frequently Asked Questions

What is the catalog number for Vimentin (Mesenchymal Cell Marker) (V9), CF514 conjugate, 0.1mg/mL?

The catalog number for Vimentin (Mesenchymal Cell Marker) (V9), CF514 conjugate, 0.1mg/mL is BNC142775-100.

What sizes are available for Vimentin (Mesenchymal Cell Marker) (V9), CF514 conjugate, 0.1mg/mL?

Vimentin (Mesenchymal Cell Marker) (V9), CF514 conjugate, 0.1mg/mL is available in the following size(s): 1x 100 µL.

Is Vimentin (Mesenchymal Cell Marker) (V9), CF514 conjugate, 0.1mg/mL in stock?

Vimentin (Mesenchymal Cell Marker) (V9), CF514 conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Vimentin (Mesenchymal Cell Marker) (V9), CF514 conjugate, 0.1mg/mL?

Vimentin (Mesenchymal Cell Marker) (V9), CF514 conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for Vimentin (Mesenchymal Cell Marker) (V9), CF514 conjugate, 0.1mg/mL?

Estimated delivery time for Vimentin (Mesenchymal Cell Marker) (V9), CF514 conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Vimentin (Mesenchymal Cell Marker) (V9), CF514 conjugate, 0.1mg/mL?

The safety data sheet for Vimentin (Mesenchymal Cell Marker) (V9), CF514 conjugate, 0.1mg/mL is available here: https://cdn.gentaur.com/products/37/68dd029b061270361e101d90/msds/bnc142775-100.pdf.

What is Vimentin (Mesenchymal Cell Marker) (V9), CF514 conjugate, 0.1mg/mL used for?

This MAb reacts with a 58 kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Anti-vimentin alone is of limited value as a diagnostic tool; however,

Does this cell line or biologic require a Material Transfer Agreement (MTA)?

Some products, including certain cell lines, plasmids, and tissue cultures, are subject to intellectual property terms and may require an MTA before we can ship. We’ll inform you in advance if this product requires one, and additional handling fees may apply.

What should I do if I need to report a performance issue with a specific lot of this product?

Provide the catalogue number, product name, lot number, and your experimental results to our technical support team. They’ll investigate and, if needed, escalate to the manufacturer, provided the product was used before expiry and stored correctly.

What is the isotype of this antibody?

Isotype information such as IgG1 or IgG2a is listed in the product specifications on the datasheet, relevant for selecting compatible detection reagents.

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