Cig2 (3A11/5), CF488A conjugate, 0.1mg/mL

About this product

Cig2 is a cyclin that functions as the catalytic subunit of cyclin-dependent kinases (Cdks) in the fission yeast Schizosaccharomyces pombe. It is a B-type, S phase cyclin that is required for both the G1/S and G2/M cell cycle transitions and is expressed in a sharp spike that peaks during the G1/S period. Cig2 binds to Cdc2 (Cdk), and the resulting Cdc2/Cig2 complex controls the G1/S transition of the cell cycle. Disruption of Cig2 delays the onset of mitosis. The expression of the Cig2 gene is dependent on Mlu1-binding factor (MBF), and the protein is destroyed during anaphase by the APC/cyclosome (APC/C) and Skp1/Cullin-1/F-box (SCF), thereby ensuring the spike expression pattern of Cig2. SCF regulates Cig2 levels in a dual manner, transcriptionally and post-translationally, while APC/C only destroys the protein. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC882827-500

Size / count

1x 500 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

B type S phase cyclin antibody

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

Not Known

Gene ID

Not Known

NCBI Gene ID

Not Known

UniProt

Not Known

Swiss Prot

Not Known

Host

Mouse

Species Reactivity

Yeast

Reactivity

Cig2

Immunogen

Bacterial S. pombe Cig2 Purified protein.

Target Antigen

Cig2

Clonality

Monoclonal

Clone

3A11/5

Type

Primary

Source

Animal

Origin

Animal

Positive Control

Red blood cell lysate; Human colon and lung tissue.

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

~50 kDa

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG1, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF488A