SIGLEC1 / CD169 / Sialoadhesin (HSn 7D2), CF647 conjugate, 0.1mg/mL

About this product

Two families of mammalian lectin-like adhesion molecules, the selectins and the sialoadhesins, bind glycoconjugate ligands in a sialic acid-dependent manner. The sialic acid-binding immunoglobulin superfamily lectins, designated siglecs or sialoadhesins, are immunoglobulin superfamily members that recognize sialylated ligands. The common sialic acids of mammalian cells are N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc) . The human Siglec-1 gene maps to chromosome 20p13 and encodes a 1, 709 amino acid protein, also known as CD169. Alternative splicing of the Siglec-1 gene produces a variant, encoding a type I transmembrane protein isoform that is soluble rather than membrane-bound. Studies have shown human Siglec-1 has greater affinity for Neu5Ac over Neu5Gc. Siglec-1 is a sialic acid-binding receptor that is expressed in hemopoietic cells. It mediates local cell-cell interactions in lymphoid tissues and can be detected at contact points of macrophages with other macrophages, sinus-lining cells and reticulum cells. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC472843-100

Size / count

1x 100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

CD169; CD169 antigen; Sialic acid binding immunoglobulin like lectin 1; Sialic acid-binding Ig-like lectin 1; Sialoadhesin; Siglec-1; SN

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

SIGLEC1

Gene ID

6614

NCBI Gene ID

31869

UniProt

Q9ZZ2

Swiss Prot

Q9ZZ2

Host

Mouse

Species Reactivity

Human

Reactivity

CD169|Sialoadhesin|SIGLEC1

Immunogen

Fc fusion protein containing N-terminal 4 domains of human sialoadhesin.

Target Antigen

CD169 | Sialoadhesin | SIGLEC1

Clonality

Monoclonal

Clone

HSn 7D2

Type

Primary

Source

Animal

Origin

Animal

Applications

Flow, surface (published) |Functional studies (published) |IHC, FFPE (published) |WB (published)

Positive Control

Human spleen, human peripheral blood monocytes or MCF7 cell.

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

185 kDa

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. Cancer Sci (2013) 104(9): 1237-1244. (WB; cell ELISA; IHC, FFPE) Rheumatology (2014) 53(2): 250-259. (Flow, surface; functional studies)

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG1, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF647