Spastin (Sp 3G11-1), CF405S conjugate, 0.1mg/mL

About this product

The AAA protein family members share an ATPase domain and have roles in various cellular processes including intracellular motility, membrane trafficking, proteolysis, protein folding and organelle biogenesis. Spastin, a member of the AAA protein family, is a 616 amino acid protein and is involved in the function or assembly of nuclear protein complexes. The Spastin protein is expressed ubiquitously and localizes to the nucleus and the cytoplasm, where it may also be involved in microtubule dynamics. Mutations in the Spastin gene (SPAST, SPG4) cause the most common form of spastic paraplegia 4, an autosomal dominant form of hereditary spastic paraplegia (HSP) . HSPs comprise a group of inherited neurological disorders characterized by spastic lower extremity weakness due to a length-dependent, retrograde axonopathy of corticospinal motor neurons. SPAST-specific mutations account for approximately 40% of all autosomal dominant HSPs. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC042844-500

Size / count

1x 500 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

ADPSP; FSP2; Spast; Spatin; SPG4

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

SPAST

Gene ID

6683

NCBI Gene ID

468091

UniProt

Q9UP0

Swiss Prot

Q9UP0

Host

Mouse

Species Reactivity

Human, Mouse, Rat

Reactivity

Spastin

Immunogen

Recombinant full-length human Spastin protein.

Target Antigen

Spastin

Clonality

Monoclonal

Clone

Sp 3G11-1

Type

Primary

Source

Animal

Origin

Animal

Applications

IF (published) |WB (published)

Positive Control

HeLa cells. Rat brain extract.

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

52 kDa

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. J Cell Biol (2010) 189(6): 945-954. (WB) eLife (2015) 4:e05068. (IF) Oncotarget (2016) 7(21): 30820-30830. (IF)

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG2a, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF405S