- Home
- Shop
- New Categories
- New Blog
- Contact Us
- Cart
- Login
- Register
Select the image to view it full size.
Product details
Catalog number BNC402847-100
Price for BNC402847-100
Size / count 1x 100 µL
Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage; such expression vectors frequently encode hybrid fusion proteins consisting in part of prokaryotic and in part, eukaryotic specified proteins. One such system utilizes maltose binding protein (MBP), the 370 amino acid product of the E. coli mal E gene. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be Purified in a one step procedure by affinity chromatography crosslinked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. Expression systems utilizing the MBP fusion tag include pCG-806fx and pMal vectors. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
The catalog number for Maltose Binding Protein / MBP-probe (R29.6), CF640R conjugate, 0.1mg/mL is BNC402847-100.
Maltose Binding Protein / MBP-probe (R29.6), CF640R conjugate, 0.1mg/mL is available in the following size(s): 1x 100 µL.
Maltose Binding Protein / MBP-probe (R29.6), CF640R conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.
Maltose Binding Protein / MBP-probe (R29.6), CF640R conjugate, 0.1mg/mL is manufactured by Biotium.
Estimated delivery time for Maltose Binding Protein / MBP-probe (R29.6), CF640R conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.
The safety data sheet for Maltose Binding Protein / MBP-probe (R29.6), CF640R conjugate, 0.1mg/mL is available here: https://cdn.gentaur.com/products/37/68dd029a061270361e101677/msds/bnc402847-100.pdf.
Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage; such expression vectors frequently encode hybrid fusion proteins consisting in part of prokaryotic and in part, eukaryotic specified proteins. One such system utiliz
As a consumer, you have a 14-day right of withdrawal from receipt under EU consumer protection law, submitted via our withdrawal/cancellation request form. Note this applies to unused, undamaged products, and doesn’t override the expiry/storage conditions on performance-based claims.
Endotoxin testing status is noted on the datasheet where applicable, particularly for recombinant proteins intended for cell-based or functional assays. If this isn’t listed and you need confirmation, contact support with the catalogue number.
Additional data may be available on request. Contact our technical support team with the catalogue number and we’ll check what supporting documentation the supplier can provide.
