Fibronectin (Fn-3), CF405S conjugate, 0.1mg/mL

About this product

Fibronectins are disulfide-linked, dimeric glycoproteins of ~440 kDa. They possess at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Epitope of this MAb is located in the 2nd-3rd type-III repeats of fibronectin. Fibronectins are extracellular matrix glycoproteins that are essential for embryonic development. Fibronectins are also involved in cell adhesion, tissue organization, and wound healing. Fibronectins are present in basement membranes, interstitial connective tissue matrix, and blood. Cellular fibronectin is widely distributed in the stroma of many malignant tumors. This MAb reacts with human cellular fibronectin, but not plasma fibronectin. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC042848-500

Size / count

1x 500 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

Cold insoluble globulin (CIG) ; FINC; FN1; FNZ; GFND; GFND2; LETS; Migration stimulating factor (MSF) ; Ugl-Y3

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

FN1

Gene ID

2335

NCBI Gene ID

203717

UniProt

P02751

Swiss Prot

P02751

Host

Mouse

Species Reactivity

Human

Reactivity

Fibronectin

Immunogen

FR5 cells, derived by SV40 transformation of human mammary epithelial cells.

Target Antigen

Fibronectin

Clonality

Monoclonal

Clone

Fn-3

Type

Primary

Source

Animal

Origin

Animal

Applications

IF (published) |WB (published)

Field of Research

Cell adhesion, Extracellular matrix

Positive Control

SW156 cells. Kidney.

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

220 kDa (monomer) ; 440 kDa (dimer)

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. Am J Physiol Cell Physiol (2002) 282: C654-C664. (IF; WB) Meth Mol Biol (2009) https://doi.org/10.1007/978-1-59745-413-1_18 (IF) J Tissue Eng Regen Med. (2020) 14:761-773. (IF, frozen tissue sections)

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG1, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF405S