CD54 / ICAM-1 (15.2), CF647 conjugate, 0.1mg/mL

About this product

Recognizes an 85-115 kDa protein (variation with cell type), identified as intercellular adhesion molecule (ICAM-1) . It has 7 potential N-linked glycosylation sites. ICAM-1 is a single chain glycoprotein of Ig supergene family, present on unstimulated endothelial cells (EC) and on a variety of other cell types including activated fibroblasts, EC, macrophages, and lymphocytes. ICAM-1 mediates cell adhesion by binding to integrins CD11a/CD18 (leukocyte adhesion molecule, LFA-1) and to CD11b/CD18 (Mac-1) . This interaction enhances antigen-specific T-cell activation. ICAM-1 also binds to CD43 and to Plasmodium falciparum infected RBCs. ICAM-1 may also be related to progression and metastasis of tumors. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC472853-100

Size / count

1x 100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

CD54; Cell surface glycoprotein P3.58; Human rhinovirus receptor; ICAM-1; Intercellular adhesion molecule 1; Ly 47; Major group rhinovirus receptor; MALA2; MyD10; Surface antigen of activated B cells

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

ICAM1

Gene ID

3383

NCBI Gene ID

643447

UniProt

P05362

Swiss Prot

P05362

Host

Mouse

Species Reactivity

Human, Pig

Reactivity

CD54|ICAM-1

Immunogen

Human monocytes.

Target Antigen

CD54 | ICAM-1

Clonality

Monoclonal

Clone

15.2

Type

Primary

Source

Animal

Origin

Animal

Applications

Flow, surface (published) |Functional studies (published) |IF (published) |IHC, frozen (published)

Field of Research

Immunology

Positive Control

Raji cells, MOLT 4, HeLa or SW480 cells. Human tonsil.

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

85-115 kDa

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. J Leukocyte Biol (1998) 64: 519. (Flow, surface) Inv Ophthalmol Visual Sci (2003) 44: 1888-1896. (IHC, frozen) Blood (1999) 93(11): 3723-3735. (IF) J Cell Biol (1992) 116(6): 1527-1535. (functional studies)

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG1, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF647