Cyclin B1 (G2- & M-phase Cyclin) (CCNB1/2978R), CF568 conjugate, 0.1mg/mL

Catalog number BNC682978-100

Price for BNC682978-100

Size / count 1x 100 µL

About this product

Recognizes a protein of 55-62 kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNC682978-100
Size / count: 1x 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: CCNB; CCNB1; CCNB1_HUMAN; G2 Mitotic Specific Cyclin B1
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: CCNB1
Gene ID: 891
NCBI Gene ID: 23960
UniProt: P14635
Swiss Prot: P14635
Host: Rabbit
Species Reactivity: Hamster, Human, Mouse
Reactivity: Cyclin B1
Immunogen: Recombinant full-length human CCNB1 protein
Target Antigen: Cyclin B1
Clonality: Recombinant Monoclonal
Clone: CCNB1/2978R
Type: Primary
Source: Animal
Origin: Animal
Positive Control: Cell line in logarithmic growth phase. Tonsil or testicular, endometrial, prostate or ovarian carcinoma.
Stability: 2 years
Concentration: 0.1 mg/mL
Buffer: PBS, 0.1% BSA, 0.05% azide
Molecular Weight: 55-62 kDa
References & Citations: Minemoto Y et al. Exp Cell Res 262:37-48 (2001) |Garner AP et al. Oncogene 21:8089-104 (2002)
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Notes: Protect from light
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG
Species: Oryctolagus cuniculus (domestic rabbit), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification: 0.05% sodium azide (CAS 26628-22-8)
Conjugate: CF568

Frequently Asked Questions

What is the catalog number for Cyclin B1 (G2- & M-phase Cyclin) (CCNB1/2978R), CF568 conjugate, 0.1mg/mL?

The catalog number for Cyclin B1 (G2- & M-phase Cyclin) (CCNB1/2978R), CF568 conjugate, 0.1mg/mL is BNC682978-100.

What sizes are available for Cyclin B1 (G2- & M-phase Cyclin) (CCNB1/2978R), CF568 conjugate, 0.1mg/mL?

Cyclin B1 (G2- & M-phase Cyclin) (CCNB1/2978R), CF568 conjugate, 0.1mg/mL is available in the following size(s): 1x 100 µL.

Is Cyclin B1 (G2- & M-phase Cyclin) (CCNB1/2978R), CF568 conjugate, 0.1mg/mL in stock?

Cyclin B1 (G2- & M-phase Cyclin) (CCNB1/2978R), CF568 conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Cyclin B1 (G2- & M-phase Cyclin) (CCNB1/2978R), CF568 conjugate, 0.1mg/mL?

Cyclin B1 (G2- & M-phase Cyclin) (CCNB1/2978R), CF568 conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for Cyclin B1 (G2- & M-phase Cyclin) (CCNB1/2978R), CF568 conjugate, 0.1mg/mL?

Estimated delivery time for Cyclin B1 (G2- & M-phase Cyclin) (CCNB1/2978R), CF568 conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Cyclin B1 (G2- & M-phase Cyclin) (CCNB1/2978R), CF568 conjugate, 0.1mg/mL?

The safety data sheet for Cyclin B1 (G2- & M-phase Cyclin) (CCNB1/2978R), CF568 conjugate, 0.1mg/mL is available here: https://cdn.gentaur.com/products/37/68dd0298061270361e100eb8/msds/bnc682978-100.pdf.

What is Cyclin B1 (G2- & M-phase Cyclin) (CCNB1/2978R), CF568 conjugate, 0.1mg/mL used for?

Does this cell line or biologic require a Material Transfer Agreement (MTA)?

Some products, including certain cell lines, plasmids, and tissue cultures, are subject to intellectual property terms and may require an MTA before we can ship. We’ll inform you in advance if this product requires one, and additional handling fees may apply.

What should I do if I need to report a performance issue with a specific lot of this product?

Provide the catalogue number, product name, lot number, and your experimental results to our technical support team. They’ll investigate and, if needed, escalate to the manufacturer, provided the product was used before expiry and stored correctly.

What is the isotype of this antibody?

Isotype information such as IgG1 or IgG2a is listed in the product specifications on the datasheet, relevant for selecting compatible detection reagents.