TIGIT / VSTM3 / VSIG9 (Immune Checkpoint for Cancer) (TIGIT/3017), CF647 conjugate, 0.1mg/mL

About this product

TIGIT is a checkpoint inhibitor which binds with high affinity to the poliovirus receptor (PVR), causing increased IL10 secretion, decreased IL12B secretion. TIGIT binding to PVR also causes the suppression of T cell activation by promoting the generation of mature immuno-regulatory dendritic cells. It is expressed at low levels on natural killer (NK) cells, as well as peripheral memory and regulatory CD4 T cells. At the protein level, it is upregulated following the activation of these cells. Functionally, TIGIT is similar to CTLA4. The ligands for TIGIT include CD155 (signal abrogation) and CD226 (signal stimulation) . It has been demonstrated to be upregulated on T cells in many cancers and is an immuno-oncology target for therapy. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC473017-500

Size / count

1x 500 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

T-cell immunoreceptor with Ig and ITIM domains; V-set and immunoglobulin domain containing 9 (VSIG9) ; V-set and transmembrane domain containing 3 (VSTM3) ; Washington University cell adhesion molecule (WUCAM)

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

TIGIT

Gene ID

201633

NCBI Gene ID

421750

UniProt

Q4951

Swiss Prot

Q4951

Host

Mouse

Species Reactivity

Human

Reactivity

TIGIT|VSIG9|VSTM3

Immunogen

Recombinant fragment (around aa 22-141) of human TIGIT protein (exact sequence is proprietary)

Target Antigen

TIGIT | VSIG9 | VSTM3

Clonality

Monoclonal

Clone

TIGIT/3017

Type

Primary

Source

Animal

Origin

Animal

Applications

Flow (verified) |IHC, FFPE (verified)

Positive Control

MOLT-4, Prostate or Colon Carcinoma.

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

26 kDa

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG2c, kappa

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF647